血液透析患者单个核细胞中TTV-DNA的研究

目的 :探讨血液透析患者血清和外周血单个核细胞 (PBMC)中输血传播病毒 (TTV)检测状况及意义。方法 :采用聚合酶链反应 (PCR)对 6 6例血液透析患者血清及PBMC进行TTV DNA检测 ,同时采用酶免疫分析 (EIA)检测血清中HBsAg和抗 HCV。结果 :血液透析患者TTV、HCV、HBV检出率分别为 18.2 %、2 4 .2 %、7.6 %。血清TTV DNA阳性与阴性患者 ,抗 HCV阳性率分别为 9.1%与 2 7.3% ,HBsAg阳性率分别为 0 %与 9.1% ,经统计学分析均无显著差异。PBMC中TTV DNA检出率 2 2 .7% ,PBMC中TTV DNA检出率在血清TTV DNA阳性者显著高于血清TTV DNA阴性者 (5 8.3%vs 14 .8% ,P<0 .0 5 )。结论 :血液透析患者TTV感染不依赖于HCV或HBV而存在。PBMC中TTV DNA主要在血清TTV DNA阳性患者中检出 ,PBMC可能是TTV的一个贮存场所。

Study on Transfusion Transmitted Virus- DNA in PBMC of Hemodialysis Patients

Objective: To study the situation and significance of transfusion transmitted virus (TTV) in serum and peripheral blood mononuclear cell (PBMC) of hemodialysis patients. Methods: TTV DNA was detected in serum and PBMC of 66 hemodialysis patients by polymerase chain reaction (PCR). HBsAg and Anti HCV in sera was detected by EIA. Results: Infected rate of TTV, HCV, HBV in hemodialysis patients was 18.2%,24.2%,7.6% respectively. There was no significant difference between TTV DNA positive and negative in patients with positive anti HCV (9.1% vs 27.3%) and of HBsAg (0% vs 9.1%). Positive rate of TTV DNA in PBMC was 22.7%. It was significantly higher in TTV DNA positive patients than negative patients (58.3% vs 14.8%, P< 0.05). Conclusion: TTV infection in hemodialysis patients may not rely on HCV or HBV. TTV DNA in PBMC is related with TTV DNA positive sera . PBMC may be a reservoir for TTV.