当归补血汤对内皮细胞增殖和粘附分子表达的影响

目的　了解当归补血汤对内皮细胞增殖及分泌粘附分子 (ICAM- 1)的影响 ,探讨当归补血汤对造血调控的机理。方法　建立人脐静脉内皮细胞体外培养模型 ,将 HU VEC与当归补血汤不同剂量 (0、4、40、40 0和40 0 0 μg/ m l)一起培养 ,以流式细胞分析术测定内皮细胞增殖周期 ,同时对粘附分子进行测定。结果　当归补血汤能够促进内皮细胞分泌粘附分子 ,对照组 CD5 4(ICAM- 1)仅有少量表达 ,而实验组 CD5 4(ICAM- 1)表达明显较对照组增多 (P<0 .0 5 )。细胞周期分析结果表明 ,实验组与对照组相比 ,有更多的细胞处于 S期 (P<0 .0 5 )。提示当归补血汤能够促进细胞由静止期进入增殖期。结论　当归补血汤能够促进内皮细胞的增殖以及细胞粘附分子的表达。

Effect of Danggui Buxue Decoction on Proliferation and Expression of Intercellular Adhesion Molecule-1 in Human Umbilical Vein Endothelial Cells

Objective To investigate the effect of Danggui Buxue Decoction (DBD) on proliferation and expression of intercellular adhesion molecule 1(ICAM 1) in human umbilical vein endothelial cells(HUVECs). Methods After treating HUVECs with various concentrations of DBD,we analyzed the proliferation phase of HUVECs and the expression of ICAM 1 using flow cytometry. Results It was found that DBD could promote the proliferation of HUVECs and the expression of ICAM 1 .The expression of CD54(ICAM 1) was significantly increased in the experiment groups as compared with that in the control group ( P <0.05). In addition, cell cycle analysis revealed more synthetic period cells in the experiment groups than in control group ( P <0.05). Conclusion DBD can promote the proliferation of HUVECs and Increase the expression ICAM 1.