| 双氯芬酸钠脂质体的制备及包封率测定 |
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| 引用本文： | 蔡明志,张敬如,黄复生. 双氯芬酸钠脂质体的制备及包封率测定[J]. 中国药房, 2008, 19(28): 2206-2208 |
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| 作者姓名： | 蔡明志 张敬如 黄复生 |
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| 作者单位： | 1. 解放军第一五四中心医院药剂科,信阳市,464000
2. 第三军医大学病原生物学教研室,重庆市,400038 |
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| 摘 要： | 目的:制备双氯芬酸钠脂质体并测定其包封率。方法:以pH梯度法制备脂质体,透射电子显微镜考察其形态与粒径;采用高效液相色谱法测定双氯芬酸钠的包封率。结果:制备的脂质体粒径大小均匀,粒径范围为150～175nm;双氯芬酸钠检测浓度的线性范围为0.01～1mg·mL-1(r=0.9990,n=6),平均回收率为100.05%(RSD=1.21%,n=6);包封率最高可达85.8%,最低52.4%。结论:本方法可制备得到包封率较高的双氯芬酸钠脂质体。
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| 关 键 词： | 双氯芬酸钠脂质体 高效液相色谱法 制备 包封率 |
Preparation and Encapsulation Efficiency Determination of Diclofenac Sodium Liposomes |
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| CAI Ming-zhi,ZHANG Jing-ru,HUANG Fu-sheng. Preparation and Encapsulation Efficiency Determination of Diclofenac Sodium Liposomes[J]. China Pharmacy, 2008, 19(28): 2206-2208 |
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| Authors: | CAI Ming-zhi ZHANG Jing-ru HUANG Fu-sheng |
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| Affiliation: | CAI Ming-zhi, ZHANG Jing-ru, HUANG Fu-sheng(Dept. of Pharmacy, PLA No. 154 Hospital, Xinyang 464000, China; Dept. of Pathogenic Biology, Third Military Medical University, Chongqing 400038, China) |
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| Abstract: | OBJECTIVE： To prepare diclofenae sodium liposomes and establish a method for their quality control. METHODS： The diclofenac sodium liposomes were prepared by pH gradient method; the particle shape and size were measured by transmission electrical miscroscope; the concentration and encapsulation efficiency of diclofenac sodium in the liposomes were determined by HPLC. RESULTS： The prepared lipisomes were uniformly distributed with particle size of 150--175 nm. The linear range of diclofenac sodium was 0.01-- 1 mg ·mL ^-1（r = 0.999 0, n = 6） at an average recovery of 100.05% （RSD = 1.21%, n = 6） . The encapsulation efficiency ranged from 52.4% to 85.8%. CONCLUSION： High encapsulation efficiency of diclofenac sodium liposomes can be obtained from the established method. |
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| Keywords: | Diclofenac sodium liposomes HPLC Preparation Encapsulation efficiency |
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