超高效液相色谱法测定生栀子和焦栀子药材中栀子苷含量

目的采用超高效液相色谱法(ultra performance liquid chromatography,UPLC)建立栀子药材中栀子苷的含量测定方法。方法采用BEH C18色谱柱(1.7μm,2.1mm×100mm),流动相为乙腈-水(梯度洗脱),检测波长238nm,流速为0.3ml/min,柱温35℃。结果栀子苷进样量在0.015～0.240μg范围内,与峰面积线性关系良好(y=0.056 2 x-0.075 6,r=0.999 1),生栀子的平均回收率为98.98%,相对标准偏差(relative standard deviation,RSD)为0.40%(n=5);焦栀子的平均回收率为98.12%,RSD=0.47%(n=5)。结论超高效液相色谱法简便、快速、准确、重复性好,可用于栀子药材中栀子苷的检测。

Content Determination of Geniposide in Fructus Gardeniae and Baked Fructus Gardeniae Using Ultra Performance Liquid Chromatography

Objective To establish an ultra performance liquid chromatography(UPLC) method for determining the contents of Geniposide in the medical materials of Fructus Gardeniae.Methods BEH C18 chromatographic column(1.7 μm,2.1 mm×100 mm) was employed,with acetonitrile-water(gradient elution) being mobile phase,wavelength being set at 238 nm,flow rate being 0.3 ml/min,and column temperature being 25 ℃.Results Within the range of 0.015 to 0.240 μg,Geniposide had a good linear relation to the peak area(y=0.056 2 x-0.075 6,r=0.999 1).The average recovery rate of Geniposide was 98.98% in Fructus Gardeniae(relative standard deviation was 0.40%,n=5) and 98.12% in baked Fructus Gardeniae(relative standard deviation was 0.47%,n=5).Conclusion UPLC can be used for determining Geniposide in medical materials of Fructus Gardeniae Because of its simplicity,rapidness,accuracy,and perfect reproducibility.