Revised amino acid sequences of the three major phospholipases A2 from Bungarus fasciatus (banded krait) venom

C.-S. , J.-M. , C.-H. , S.-W. , I.-H. , H.-S. and T.-B. . Revised amino acid sequences of the three major phospholipases A₂ from Bungarus fasciatus (banded krait) venom. Toxicon 28, 1457–1468, 1990.—The structures of three cardiotoxin-like proteins obtained from the venom of Bungarus fasciatus (banded krait) were elucidated previously ( and , 1980, 1981). Since their molecular sizes are similar to that of phospholipase A₂ and since they show weak phospholipase A₂ activities ( et al., 1983), a further study of their primary structures was carried out. Fractions Va, Vb-2 and VI, corresponding to the former V-2, V-3 and VI were determined to be typical phospholipases A₂. Among 118 amino acid residues, they all have in common a Pro 29 between Gly 28 and Gly 30, the latter two residues being implicated in Ca²⁺ binding together with Tyr 26 and Asp 47.