苦参碱诱导骨肉瘤细胞凋亡的实验研究

目的：探讨苦参碱对人骨肉瘤细胞的生长抑制和诱导凋亡作用。方法：将不同浓度的苦参碱作用于培养的OS732骨肉瘤细胞，通过MTT比色法、流式细胞仪和DNA凝胶电泳、电子显微镜技术观察检测细胞凋亡，研究分析其对骨肉瘤细胞的生长抑制和诱导凋亡作用。结果：苦参碱浓度为0．5、1．0、1．5g／l时，细胞毒性指数分别为12．1％、21．6％、39．5％；流式细胞术示骨肉瘤细胞的凋亡率分别为9．8％、16．9％、25．5％；DNA电泳见DNA“梯形”图谱；电镜显示细胞的凋亡形态。结论：苦参碱能显著抑制OS732细胞增殖、促进其凋亡。

Experimental study on apoptosis of osteosarcoma cells induced by matrine

Objective To study the growth-inhibition and the mechanism of apoptosis induced by matrine on OS732 osteosarcoma cells.Methods Osteosarcoma cells were treated with matrine for 3 days.The cytotoxicity was observed by MTT assay.The apoptotic rates were shown by flow cytometry(FCM).Electron microscopy and DNA gel electrophoresis were applied to demonstrate the presence of apoptosis.Results When osteosarcoma cells treated with matrine (0.5,1.0 and 1.5g/L),the cytotoxicity index were 12.1%,21.6% and 39.5% respectively,the apoptostic rates were 9.8%,16.9% and 25.5%.The obvious apotosis morphological changes in osteosarcoma cells were shown by electron microscope.Conclusion Matrine is able to inhibite the proliteration and induced the apoptosis of OS732 cells significantly.It is apotential and valuable proposal for clicial treatment of osteosarcoma.