急性一氧化碳中毒后大鼠脑白质中LINGO-1的动态表达变化

目的：观察LINGO-1在急性一氧化碳（CO）中毒后大鼠脑白质中的动态表达变化。方法：选择70只SD雄性大鼠随机分为对照组，染毒后1d、3d、7d、14d、21d、28d组（n-10）。采用甲苯胺蓝染色观察染毒后各时间点大鼠脑白质髓鞘脱失情况，并应用免疫组织化学染色及实时定量RT-PCR的方法观察LINGO-1的表达变化。结果：①对照组LINGO-1表达极低，染毒后1d，LINGO-1mRNA和蛋白表达上调均最为显著，3d时下降接近至对照组水平，在7d时均再次出现上调至21d开始下降，28d时LINGO-1mRNA接近对照组水平，而LINGO-1蛋白略高于对照组。②对照组无脑白质脱髓鞘出现，染毒后7d观察到脑白质轴突髓鞘脱失，直至28d脱髓鞘现象仍在发生。结论：在大鼠发生急性CO中毒后，其脑白质中的LNGO-l即发生上调，并呈现二次上调的规律性改变，且二次上调与脑白质脱髓鞘病变发生时间相关联，提示其与CO中毒后脑白质脱髓鞘病变相关。

Dynamic expression of LINGO-1 in the cerebral white matter of rats after acute CO- poisoning

Objective：To investigate the dynamic expression of LINGO-1 in the cerebral white matter of acute carbon monoxide （CO） poisoned rats. Method： 70 SD male rats were randomly assigned to control group and CO exposure for 1 d,3 d,7 d,14 d,21 d,28 d groups,and ten rats for each group. Quantitative real-time PCR （qRT- PCR） and immunohistochemical staining were performed to study the expression of LINGO-1 in the cerebral white matter. Demyelinated areas in the cerebral white matter were detected by toluidine blue staining. Result： The ex- pression of LINGO 1 mRNA and protein were very low at control group, and it increased in-CO exposure. The peak time was 1 d post--poisoning and decreased to normal level after 3 days. After 7 days, the LINGO-1 mRNA increased again and decreased to normal level until 28 days. The LINGO-1 protein decreased slowly and was still higher after 4 weeks. Demyelinated axtons were detected at 1 week after CO exposure and still increased at 4 weeks after CO exposure. Conelusion： These results show the higher expression of LINGO-1 in the acute stage time of CO exposure and the second increase after the demyelinating time. Our findings demonstrate LINGO-1 may be involved in the happening of demyelination after acute CO poisoning.