圆锥角膜组织中EGF和bFGF变化免疫组织化学的研究

目的 观察正常人及圆锥角膜中表皮生长因子(EGF)和碱性成纤维细胞生长因子（bFGF)的表达，探讨EGF和hFGF在圆锥角膜中的变化。方法 采用免疫组织化学SABC法检测角膜组织中EGF、bFGF的表达，结果 在圆锥角膜上皮层、Bowman层和内皮层有EGF表达，EGF比正常角膜的表达强；在圆锥角膜的上皮层、实质层和内皮层可见bFGF的阳性表达，圆锥角膜的基质瘢痕区bFGF有较强的阳性表达。结论 圆锥角膜组织中EGF水平增高，促进上皮细胞和基质细胞的DNA合成，从而使组织愈合。bFGF表达增强可以促进角膜上皮、基质细胞和内皮细胞的增殖。

Immunohistochemistry of EGF and bFGF in keratoconus

ObjectiveTo observe the expression of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) in normal cornea and keratoconus and discuss their effects on keratoconus.MethodsTwenty one keratoconuses and 7 normal corneas were detected.Immunohistochemical SABC method was used to detect the expression of EGF and bFGF in cornea.ResultsEGF was expressed in epithelium, endothelium and Bowman layer in normal cornea and keratoconus but the positive expression increased in keratoconus.The bFGF was expressed in epithelium,endothelium and stroma of cornea.The strong positive expression was found in the scar tissue in keratoconus stroma.ConclusionIncreasing EGF accelerates DNA synthesis of epithelial cells and stromal cells in keratoconus to cause tissue healing.High level of bFGF in keratoconus accelerate the proliferation of epithelium,stroma and endothelium.