幽门螺杆菌L型感染与胃癌血管形成的关系

背景与目的:研究幽门螺杆菌L型(helicobacter pylori L-form,Hp-L)感染与胃癌的关系及对胃癌血管形成的影响,并探讨其机制.材料与方法:应用革兰染色和免疫组化SP法检测130例胃癌组织和50例对照组的Hp-L型感染;同时应用免疫组化检测上述组织的CD34表达,计算微血管密度(microvessel density,MVD),分析Hp-L型与MVD以及临床病理因素的关系.结果:胃癌组织中革兰染色L型检出阳性率为70.00%(91 130);免疫组化Hp-L型抗原表达阳性率为73.85%(96 130),两种方法检测同时阳性的病例88例,占67.69%.对照组中革兰染色L型检出阳性14例,免疫组化Hp-L型抗原表达阳性17例,两种方法同时阳性12例,其阳性率是24.00%(12 50),胃癌组与对照组的Hp-L型阳性率相比差异有统计学意义(P<0.05).胃癌组中MVD值高于对照组,差异有统计学意义(P<0.05);np-L型感染阳性的胃癌中,MVD值与肿瘤的大小无关,但与癌细胞的血管侵袭、侵袭深度、局部和远处淋巴结转移相关(P<0.05).结论:Hp-L感染与胃癌的发生密切相关,Hp-L感染可能是肿瘤血管生成的重要促进因子,影响胃癌的侵袭和转移.

Relation between Helicobacterpylori L-form Infection andAngiogenesis in GastricCarcinoma

BACKGROUND AND AIM: To investigate the correlation between infection of helicobacter pylori L-form(Hp-L) and gastric carcinoma,and to investigate the effect of Hp-L on angiogenesis of gastric carcinoma.MATERIALS AND METHODS: Hp-L was examined in 130 patients with gastric carcinoma and 50 control group by Gram staining and immunohistochemical stain SP method.The latter method was also used to detect the CD34 expression to measure MVD,and their relationship with clinicopathologic factors were analyzed.RESULTS: The positive rates for Hp-L form in gastric carcinoma was 70.00%(91/130) by Gram staining,which was similar to the positive rate(73.85%) by immunohistochemical stain(P>0.05).Hp-L infection rate in gastric carcinoma was much higher than in control group(67.69% vs 24.00%,P<0.05).MVD in gastric carcinoma was significantly higher than control groups(P<0.05).The MVD in Hp-L positive gastric carcinoma correlated with vessel invasion,depth of invasion,metastasis to the paragastric and distant lymph nodes but not with tumor size(P<0.05).CONCLUSION: Hp-L infection correlated with occurrence of gastric carcinoma.Hp-L infection may be an important promoting factor in angiogenesis of gastric carcinoma and related to invasion and metastasis.