黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

目的 探讨黄芩苷对辐射诱导小鼠的急性肺损伤保护作用。方法 X-线复制小鼠的急性肺损动物模型。将50只ICR小鼠随机分为空白对照组、X-线组、X-线组+地塞米松组（5mg/kg）、X-线组+黄芩苷组（20、40mg/kg）。观察各组肺组织病理学改变，测量肺湿/干质量比，支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、炎症因子白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）含量及肺组织中p-NFκBp65、p-IκBα蛋白表达。结果 黄芩苷组（20、40mg/kg）可有效减轻辐射所致肺组织病理学变化，能降低肺泡灌洗液中炎症细胞的数目，能显著降低肺湿/干质量比重，能提高SOD水平，并能降低MDA水平，降低p-NFκBP65和p-IκBα蛋白的表达。结论 黄芩苷可减轻辐射所致急性肺组织损伤，对辐射诱导的急性肺损伤有保护作用。 

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice. METHODS 50 ICR mice were divided into five groups randomly， namely， control group， X-ray group， X-ray+dexamethasone group (5mg/kg)， X-ray+baicalin group (20， 40 mg/kg)， with 8 in each group. Pathological changes in the lung tissues were observed. The ratio of lung wet/dry weight was also recorded. Besides， the level of neutrophil in bronchoalveolar lavage fluid (BALF) was detected to evaluate inflammatory cell infiltration. Moreover， the expressions of MDA， IL-6， TNF-α， p-NFκBp65 and p-IκBα， as well as the activity of SOD， were examined in lung. RESULTS Baicalin (20， 40 mg/kg) could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF. The ratio of lung wet/dry weight was also decreased with the treatment of baicalin (20， 40 mg/kg). In addition， the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile. The expressions of p-NFκBp65 and p-IκBα was extensively down-regulated with baicalin (20， 40 mg/kg) preconditioning， taking the expressions of NFκBp65 and IκBα as inner controls. CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.