| 单链DNA结合蛋白1沉默对颌下腺细胞放射后增殖修复的影响 |
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| 引用本文： | 陈龙,吕秋丽,义彬玲,孙喆,王代友. 单链DNA结合蛋白1沉默对颌下腺细胞放射后增殖修复的影响[J]. 中华放射医学与防护杂志, 2017, 37(9): 645-650 |
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| 作者姓名： | 陈龙 吕秋丽 义彬玲 孙喆 王代友 |
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| 作者单位： | 530021 南宁, 广西医科大学附属口腔医院口腔颌面外科 广西口腔颌面修复与重建研究重点实验室 广西颌面外科疾病诊治研究高校重点实验室,530021 南宁, 广西医科大学附属口腔医院口腔颌面外科 广西口腔颌面修复与重建研究重点实验室 广西颌面外科疾病诊治研究高校重点实验室,530021 南宁, 广西医科大学附属口腔医院口腔颌面外科 广西口腔颌面修复与重建研究重点实验室 广西颌面外科疾病诊治研究高校重点实验室,530021 南宁, 广西医科大学附属口腔医院口腔颌面外科 广西口腔颌面修复与重建研究重点实验室 广西颌面外科疾病诊治研究高校重点实验室,530021 南宁, 广西医科大学附属口腔医院口腔颌面外科 广西口腔颌面修复与重建研究重点实验室 广西颌面外科疾病诊治研究高校重点实验室 |
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| 基金项目： | 国家自然科学基金（81560187）；广西自然科学基金重点项目（2014GXNSFDA118022）。 |
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| 摘 要： | 目的 研究沉默大鼠颌下腺（submandibular gland，SMG）细胞单链DNA结合蛋白1（single-strand DNA-binding protein 1，SSB1）表达后，放射损伤下细胞的增殖及修复情况。方法 机械胰酶联合法进行大鼠SMG细胞原代培养及免疫组织化学法鉴定。实验分3组：正常细胞组、阴性对照组（Ad-HK组）和实验组（Ad-SSB1-shRNA组）。重组腺病毒介导shRNA转染SMG细胞，荧光定量PCR检测SSB1表达沉默情况。CCK-8法及免疫荧光法分别检测细胞活性及γ-H2AX焦点数动态变化。结果 细胞免疫组织化学检测角蛋白和α-淀粉酶表达均呈阳性。转染72 h后转染效率接近90%，实验组SSB1 mRNA表达较正常细胞组明显降低（t=16.24，P<0.05）。照射前，实验组细胞活性下降不明显，120 h时细胞活性才明显低于正常细胞组（t=3.29，P<0.05）。5 Gy照射后，实验组各时间点细胞活性均明显低于阴性对照组和正常细胞组（F=10.19~30.13，P<0.05）。γ-H2AX免疫荧光显示，沉默SSB1能抑制细胞DNA放射损伤修复。结论 沉默SSB1表达的大鼠SMG细胞对放射损伤更敏感。SSB1在大鼠SMG细胞DNA放射损伤修复中有着重要作用。
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| 关 键 词： | 单链DNA结合蛋白1 颌下腺细胞 电离辐射 DNA双链断裂 |
| 收稿时间： | 2017-01-13 |
Effects of silencing single-stranded DNA-binding protein 1 gene on proliferation and DNA repair of submandibular gland cells after irradiation |
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| Chen Long,Lyu Qiuli,Yi Binling,Sun Zhe and Wang Daiyou. Effects of silencing single-stranded DNA-binding protein 1 gene on proliferation and DNA repair of submandibular gland cells after irradiation[J]. Chinese Journal of Radiological Medicine and Protection, 2017, 37(9): 645-650 |
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| Authors: | Chen Long Lyu Qiuli Yi Binling Sun Zhe Wang Daiyou |
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| Affiliation: | Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Key Laboratory of Guangxi Oral and Maxillofacial Prosthetics and Reconstruction Research, Key Laboratory of University Diagnosis and Treatment of Maxillofacial Surgery in Guangxi, Nanning 530021, China,Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Key Laboratory of Guangxi Oral and Maxillofacial Prosthetics and Reconstruction Research, Key Laboratory of University Diagnosis and Treatment of Maxillofacial Surgery in Guangxi, Nanning 530021, China,Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Key Laboratory of Guangxi Oral and Maxillofacial Prosthetics and Reconstruction Research, Key Laboratory of University Diagnosis and Treatment of Maxillofacial Surgery in Guangxi, Nanning 530021, China,Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Key Laboratory of Guangxi Oral and Maxillofacial Prosthetics and Reconstruction Research, Key Laboratory of University Diagnosis and Treatment of Maxillofacial Surgery in Guangxi, Nanning 530021, China and Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Key Laboratory of Guangxi Oral and Maxillofacial Prosthetics and Reconstruction Research, Key Laboratory of University Diagnosis and Treatment of Maxillofacial Surgery in Guangxi, Nanning 530021, China |
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| Abstract: | |
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| Keywords: | Single-stranded DNA-binding protein 1 Submandibular gland cell Ionizing radiation Double-stranded DNA breaks |
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