| 塔斯品碱衍生物对人肝癌SMMC7721细胞生长的抑制作用研究 |
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| 引用本文： | Zhang YM,Wang N,Dai BL,He LC. 塔斯品碱衍生物对人肝癌SMMC7721细胞生长的抑制作用研究[J]. 中药材, 2011, 34(7): 1094-1097 |
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| 作者姓名： | Zhang YM Wang N Dai BL He LC |
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| 作者单位： | 西安交通大学医学院; |
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| 基金项目： | 国家自然科学基金面上项目(81001447); 陕西省自然科学基金(2010JQ4017) |
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| 摘 要： | 目的:分析探讨塔斯品碱衍生物对人肝癌SMMC7721细胞的生长抑制作用,研究其作用机制。方法:采用MTT法测定5种塔斯品碱衍生物对肝癌SMMC7721细胞生长的抑制作用,流式细胞术检测Tas-D1对肝癌SMMC7721细胞周期的影响,ELISA法测定Tas-D1对肝癌SMMC7721细胞EGF和VEGF蛋白的影响,PCR法测定Tas-D1对EGF和VEGF的mRNA水平的影响。结果:塔斯品碱衍生物Tas-D1对SMMC7721细胞生长具有明显的抑制作用,使细胞阻滞在S期,呈现出良好的剂量依赖性。ELISA法和RT-PCR法结果表明Tas-D1对SMMC7721细胞的EGF和VEGF蛋白质的表达和mRNA的表达有一定的抑制作用(P<0.05)。结论:塔斯品碱衍生物Tas-D1能抑制人肝癌SMMC7721细胞的生长,转换细胞周期,初步作用机制主要为Tas-D1抑制SMMC7721细胞EGF和VEGF的表达。
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| 关 键 词： | 塔斯品碱衍生物 SMMC7721细胞 生长抑制 表达 |
Effect of taspine derivatives on human liver cancer SMMC7721 |
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| Zhang Yan-min,Wang Nan,Dai Bing-ling,He Lang-chong. Effect of taspine derivatives on human liver cancer SMMC7721[J]. Journal of Chinese medicinal materials, 2011, 34(7): 1094-1097 |
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| Authors: | Zhang Yan-min Wang Nan Dai Bing-ling He Lang-chong |
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| Affiliation: | School of Medcine, Xi'an Jiaotong University, Xi'an 710061, China. zhang2008@mail.xjtu.edu.cn |
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| Abstract: | Objective:To analyse the inhibition effect of taspine derivatives on human Liver cancer SMMC7721 cell and its mechanism.Methods:The effects of five taspine derivatives on SMMC7721 cell growth were determined by MTT.The flow cytometry was used to determine the cell cycle.The effects of Tas-D1 on the EGF and VEGF in SMMC7721 cell were determined by ELISA.The mRNA level of EGF and VEGF in SMMC7721 cell was determined by RT-PCR.Results:The MTT assay demonstrated that the taspine derivative Tas-D1 significantly ... |
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| Keywords: | Taspine derivatives SMMC7721 Growth inhibition Expression |
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