| 通关藤及其混淆品的RAPD鉴别研究 |
| |
| 作者姓名： | Zhang H Pei ZD Ni C Kang TG |
| |
| 作者单位： | 辽宁中医药大学药学院; |
| |
| 基金项目： | 中国博士后科学基金特别资助项目(200902550); 辽宁省教育厅基金项目(2009T006); 辽宁省科技厅博士启动基金项目(20101069) |
| |
| 摘 要： | 目的:应用RAPD技术鉴别通关藤及其混淆品,并分析同种不同产地通关藤基因同源性。方法:CTAB法提取7个不同产地的通关藤及其6种混淆品的DNA,以20条随机引物进行非特异扩增。结果:随机引物285(GGG AAC CCG T)可稳定扩增不同产地通关藤药材的DNA,随机引物E01(CCC AAG GTC C)可有效的鉴别通关藤及其混淆品。结论:RAPD法可准确鉴别正品通关藤及其混淆品,不同产地通关藤的基因具有同源性。
|
| 关 键 词： | 通关藤 混淆品 RAPD 鉴别 |
Identification of marsdeniae tenacissimae caulis and its adulterants by RAPD |
| |
| Zhang H,Pei ZD,Ni C,Kang TG.Identification of marsdeniae tenacissimae caulis and its adulterants by RAPD[J].Jorunal of Chinese Medicinal Materials,2011,34(9):1355-1357. |
| |
| Authors: | Zhang Hui Pei Zhi-Dong Ni Chong Kang Ting-Guo |
| |
| Institution: | ZHANG Hui,PEI Zhi-dong,NI Chong,KANG Ting-guo(Liaoning Universitiy of Chinese Traditional Medicine,Dalian 116600,China) |
| |
| Abstract: | Objective:To identify Marsdeniae Tenacissimae Caulis and its adulterants by RAPD(random amplified polymorphic DNA) and analyze the gene homology of Marsdeniae Tenacissimae Caulis from various habitats. Methods:General DNA was isolated from Marsdeniae tenacissimae Caulis which were from seven various habitats and its six adulterants by CTAB,the twenty RAPD was used to identify them.Results:Random primer 285(GGG AAC CCT T)could amplify the gene of Marsdeniae tenacissimae Caulis from various habitats stablely,... |
| |
| Keywords: | Marsdeniae Tenacissimae Caulis Adulterants RAPD Identification |
| 本文献已被 CNKI PubMed 等数据库收录！ |
