| 瓜馥木总黄酮提取纯化及其清除自由基活性研究 |
| |
| 作者姓名： | Fu CY Liu YH Chen DW Tang H Feng F Zhou ZL |
| |
| 作者单位： | 邵阳医学高等专科学校;湖南省新邵县红十字会医院;北京中医药大学中药学院; |
| |
| 基金项目： | 湖南省教育厅科研基金(10C0294); 邵阳医学高等专科学校基金(XK200805) |
| |
| 摘 要： | 目的:研究瓜馥木总黄酮最佳提取工艺,并将总黄酮进行富集纯化,测试其清除自由基活性。方法:采用正交实验,考察乙醇浓度、提取温度、固液比及提取时间4个因素对该工艺的影响;利用AB-8大孔吸附树脂分离纯化总黄酮粗品;采用清除DPPH自由基初步评价瓜馥木提取物及总黄酮的抗氧化能力。结果:确立了瓜馥木总黄酮的最佳提取工艺为:提取时间3 h,乙醇浓度90%,固液比1∶10,提取温度为70℃。黄酮粗品经AB-8大孔树脂纯化以后,含量可达到55%以上。结论:优选出的最佳提取工艺条件及纯化总黄酮的方法对进一步的工艺开发有较好的参考价值,瓜馥木富集纯化的总黄酮部位具有较强的清除自由基能力,为开发成为中药保健品提供了一定的依据。
|
| 关 键 词： | 瓜馥木 总黄酮 提取工艺 清除自由基 纯化 |
Study on extraction and purification techniques and free radical scavenging activity of total flavonoids from Fissistigma oldhamii |
| |
| Fu CY,Liu YH,Chen DW,Tang H,Feng F,Zhou ZL.Study on extraction and purification techniques and free radical scavenging activity of total flavonoids from Fissistigma oldhamii[J].Jorunal of Chinese Medicinal Materials,2011,34(3):446-449. |
| |
| Authors: | Fu Chun-Yan Liu Yong-Hui Chen Dai-Wu Tang Hong Feng Fen Zhou Zhong-Liu |
| |
| Institution: | FU Chun-yan1,LIU Yong-hui2,CHEN Dai-wu1,TANG Hong1,FENG Fen1,ZHOU Zhong-liu3(1.Department of Pharmacy,Shaoyang Junior Medicine College,Shaoyang 422000,China,2.Red Cross Hospital at Xinshao County of Hunan Province,3.School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China) |
| |
| Abstract: | Objective:To optimize the extraction and purification techniques of total flavonoids in Fissistigma oldhamii.The scavenging activity to DPPH of the total flavonoids and the extraction were studied in vitro.Methods:By orthogonal design,we investigated the effects of concentration of ethanol,temperature,ratio of solid to liquid and time of extraction on the total flavonoids extraction rate.The total flavonoids was separated and purified by AB-8 macroporous resin absorption.Results:The optimum extraction condi... |
| |
| Keywords: | Fissistigma oldhamii(Hemsl )Merr Total flavonoids Extracting process Free radical scavenging activity Purification |
| 本文献已被 CNKI PubMed 等数据库收录！ |
|
