| 铁皮石斛原球茎的诱导与增殖影响因素研究 |
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| 引用本文： | 张桂芳,关杰敏,黄松,刘宏源,刘星华,朱彩霞,赖小平.铁皮石斛原球茎的诱导与增殖影响因素研究[J].中药材,2011(8). |
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| 作者姓名： | 张桂芳 关杰敏 黄松 刘宏源 刘星华 朱彩霞 赖小平 |
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| 作者单位： | 广州中医药大学;广东永生源生物科技有限公司; |
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| 基金项目： | 国家自然科学基金(81001601); 广东省自然科学基金(9451040701003210); 国家科技支撑计划(2011BA101B02) |
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| 摘 要： | 目的:研究铁皮石斛原球茎诱导与增殖的最适培养基配方。方法:以铁皮石斛种胚为材料,以MS培养基为基本培养基,研究不同植物激素6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)、2,4-D(2,4-二氯苯氧乙酸)和激动素(KT)或其组合及马铃薯提取液(PE)、香蕉提取液(BE)、苹果提取液(AE)和椰乳(CM)等有机添加物对铁皮石斛原球茎诱导和增殖的影响。结果:6-BA与NAA配比不利于种胚萌发,萌发度均低于MS培养基;添加10%的PE、CM有利于原球茎诱导;2,4-D对原球茎的生长和增殖不利,一定浓度的BA、KT和NAA有利于原球茎的增殖;KT浓度为1.0 mg/L时,原球茎40 d增殖倍数最高,达9.0;PE、CM、AE都能不同程度的促进原球茎的增殖,以10%的CM效果最好。结论:适宜铁皮石斛原球茎的诱导培养基为MS+10%CM+1.0 g/L AC;适宜铁皮石斛原球茎增殖的培养基为MS+1.0mg/L KT+0.2mg/L NAA+10%CM。
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| 关 键 词： | 铁皮石斛 原球茎 诱导 增殖 |
Study on PLB Induction and Proliferation of Dendrobium officinale |
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| ZhANG Gui-fang,GUAN Jie-min,HUANG Song,LIU Hong-yuan,LIU Xing-hua,ZHU Cai-xia,LAI Xiao-ping.Study on PLB Induction and Proliferation of Dendrobium officinale[J].Jorunal of Chinese Medicinal Materials,2011(8). |
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| Authors: | ZhANG Gui-fang GUAN Jie-min HUANG Song LIU Hong-yuan LIU Xing-hua ZHU Cai-xia LAI Xiao-ping |
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| Institution: | ZhANG Gui-fang1,GUAN Jie-min1,HUANG Song1,LIU Hong-yuan2,LIU Xing-hua2,ZHU Cai-xia1,LAI Xiao-ping1(1.Guangzhou University of Chinese Medicine,Guangzhou 510006,China,2.Guangdong Yongshengyuan Biotechnology Limited Company,Raoping 515736,China) |
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| Abstract: | Objective: To study on the optimal medium ingredients for PLB induction and proliferation of Dendrobium officinale.Methods: Seed embryos of Dendrobium officinale were cultivated in MS medium as the basic medium,along with different plant hormones like 6-BA,NAA,2,4-D and KT or their combinations added with organic additives like PE,BE,AE and CM.Results: BA and NAA combination was not conductive to germination and the germination ratio was even lower than that of MS medium;10% PE and CM was beneficial to PLB ... |
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| Keywords: | Dendrobium officinale Kimura et Migo PLB Induction Proliferation |
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