| 毛细管电泳法测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量 |
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| 作者姓名： | Yu LP Wang XK Luo JB |
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| 作者单位： | 南方医科大学中医药学院;广东药学院; |
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| 基金项目： | 国家自然科学基金资助项目(30772756) |
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| 摘 要： | 目的:建立测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱含量的毛细管电泳法。方法:采用未涂层弹性融硅石英毛细管柱(60 cm×55μm ID,有效长度52 cm);以60 mmol/L硼砂+10%(体积分数)甲醇(pH 9.0)为运行缓冲液;分离电压12 kV;重力进样10 s(高度15 cm);检测波长210 nm;以盐酸小檗碱为内标。结果:盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱浓度分别在20.0~160.0μg/mL(r=0.9999)、7.5~60.0μg/mL(r=0.9991)、2.0~10.0μg/mL(r=0.9993)内线性关系良好,3种成分的平均加样回收率依次为98.0%、97.0%、97.8%,方法精密度(RSD)依次为2.31%、2.21%、2.00%(n=6)。结论:本方法简便、快速,结果准确可靠,可用于麻杏石甘汤的质量控制。
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| 关 键 词： | 毛细管电泳法 麻杏石甘汤 盐酸麻黄碱 盐酸伪麻黄碱 盐酸甲基麻黄碱 |
Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE |
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| Yu LP,Wang XK,Luo JB.Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE[J].Jorunal of Chinese Medicinal Materials,2011,34(4):620-623. |
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| Authors: | Yu Li-ping Wang Xiao-ke Luo Jia-bo |
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| Institution: | YU Li-ping1,WANG Xiao-ke2,LUO Jia-bo1(1.School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China,2.Guangdong Pharmaceutical College,Guangzhou 510006,China) |
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| Abstract: | |
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| Keywords: | Capillary electrophoresis Maxingshigan decoction Ephedrine hydrochloride Pseudoephedrine hydrochloride Methylephedrine hydrochloride |
| 本文献已被 CNKI PubMed 等数据库收录！ |
