滇杠柳扦插繁殖及生根相关理化特性的动态分析

目的:探索不同生长素类调节剂对滇杠柳扦插繁殖的影响,以及其生根过程中内源激素、氧化酶活性的动态变化规律。方法:采用不同浓度的吲哚丁酸(IBA)、生根粉1号(ABT1)和萘乙酸(NAA)处理滇杠柳插穗,检测生根效果及生根过程中内源激素吲哚乙酸(IAA)、玉米素核苷类(ZR s)、脱落酸(ABA)含量及吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、过氧化物酶(POD)活性的变化。结果:150 mg/L IBA处理滇杠柳插穗生根率达到80%,150 mg/L ABT1和NAA处理的插穗生根率为70%和68%,而对照只有23%生根。滇杠柳扦插生根过程可分为不定根诱导期、露白期和生长期3个阶段。不定根诱导期需要低含量的内源激素IAA、ZR s、ABA、较高活性的IAAO;不定根形成露白期需要高含量内源激素IAA、较高活性的PPO、低含量的ZR s、ABA和低活性的IAAO和POD;不定根生长期需要一定含量的IAA、ZR s、ABA和较高活性的PPO、POD、IAAO,在生根后期三种氧化酶活性下降。生根过程中,150 mg/L IBA的处理增加了插穗内IAA含量和PPO活性,降低了插穗内ABA、ZR s含量和IAAO...

Cutting propagation of Periploca forrestii and dynamic analyses of physiological and biochemical characteristitics related to adventitious roots formation

Objective:To study the effects of different auxcin regulators on rooting of Periploca forrestii cuttings as well as dynamic change rules of endogenous plant hormones and oxidases related to adventitious root formation.Methods:Cuttings propagation characters of Periploca forrestii were investigated and compared with different concentration treatments of indolebutyric acid(IBA),Rooting Powder No.1(ABT1) and naphthylacetic acid(NAA).The dynamic changes of contents of endogenous hormones including indole acetic...