| 鲍氏针层孔菌菌丝体多糖脱蛋白方法研究 |
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| 作者姓名： | Xie LY Zhang Y Peng WH Gan BC |
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| 作者单位： | 四川省农业科学院土壤肥料研究所;电子科技大学生命科学与技术学院; |
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| 基金项目： | 四川省青年基金项目(2008ZQ026-072); 四川省科技支撑计划项目(2008FZ0157) |
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| 摘 要： | 目的:对多糖提取液中的蛋白质去除方法和条件进行了优化,比较不同方法对蛋白质去除的影响。方法:采用Sevag法与三氯乙酸(TCA)法去除鲍氏针层孔菌多糖中的游离蛋白质。结果:TCA去除蛋白质的效果优于Sevag法,TCA最佳脱蛋白条件为:5%三氯乙酸用量,处理3次,反应时间30min;在此条件下,蛋白质去除率为82%,多糖损失率为10.8%。结论:TCA法为最佳的鲍氏针层孔菌脱蛋白方法。
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| 关 键 词： | 鲍氏针层孔菌 Sevag法 TCA法 多糖 蛋白质去除率 多糖损失率 |
Study on methods of deproteinization from Phellinus baumii polysaccharide |
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| Xie LY,Zhang Y,Peng WH,Gan BC.Study on methods of deproteinization from Phellinus baumii polysaccharide[J].Jorunal of Chinese Medicinal Materials,2011,34(2):293-295. |
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| Authors: | Xie Li-yuan Zhang Yong Peng Wei-hong Gan Bing-cheng |
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| Institution: | XIE Li-yuan1,ZHANG Yong2,PENG Wei-hong1,GAN Bing-cheng1(1.Institute of Soil and Fertilizer,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China,2.School of Life Sciences and Technology,University of Electronic Science and Technology of China,Chengdu 610054,China) |
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| Abstract: | Objective:To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide.Methods:Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii,respectively.Results:The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows:30min treating time,5% trichloroacetic acid,3 times treatemt.Under these conditions the rate of p... |
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| Keywords: | Phellinus baumii Sevag method TCA method Polysaccharide Rate of protein-removed Rate of polysaccharide-lost |
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