| 原儿茶酸对新生大鼠皮质神经元存活及突起生长的影响 |
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| 引用本文： | Xue XY,Lin LF,Xiao F,Pi T,Lai YC,Luo HM. 原儿茶酸对新生大鼠皮质神经元存活及突起生长的影响[J]. 中药材, 2011, 34(4): 567-572 |
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| 作者姓名： | Xue XY Lin LF Xiao F Pi T Lai YC Luo HM |
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| 作者单位： | 暨南大学医学院药理学系;暨南大学药学院神经药理研究室;暨南大学-香港大学脑功能与健康联合实验室; |
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| 基金项目： | 国家“211工程”建设经费资助 |
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| 摘 要： | 目的:研究原儿茶酸(protocatechuic acid,PCA)对新生大鼠皮质神经元存活及突起生长的影响。方法:体外培养新生大鼠皮质神经元,通过细胞形态学观察、神经元细胞活力检测、培养液中乳酸脱氢酶(LDH)渗漏量的测定和有突起神经元数目及平均突起长度定量分析,观察不同剂量的原儿茶酸对神经元的营养作用。结果:与溶媒对照组比较,原儿茶酸低、中、高剂量(16、32、64μmol/L)组神经元活力增强,LDH渗漏量减少,有突起的神经元数目增多,神经元突起增长,神经网络更发达,并有一定的量效关系。结论:原儿茶酸能促进大鼠皮质神经元的存活及突起生长。
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| 关 键 词： | 原儿茶酸 大鼠皮质神经元 神经营养作用 |
Neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons of newborn rat |
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| Xue Xiao-yan,Lin Lian-feng,Xiao Fei,Pi Ting,Lai Yong-chang,Luo Huan-min. Neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons of newborn rat[J]. Journal of Chinese medicinal materials, 2011, 34(4): 567-572 |
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| Authors: | Xue Xiao-yan Lin Lian-feng Xiao Fei Pi Ting Lai Yong-chang Luo Huan-min |
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| Affiliation: | XUE Xiao-yan1,LIN Lian-feng1,XIAO Fei1,PI Ting1,LAI Yong-chang1,LUO Huan-min1,2,3(1.School of Medicine,Jinan University,Guangzhou 510632,China,2.Neuroparmachology laboratory,College of Pharmacy,3.Brain Function and Health United Laboratory,Jinan University-University of Hong Kong,China) |
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| Abstract: | Objective:To study the neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons.Methods:The newborn rat cerebral cortex neurons were cultured in vitro.The convert phase microscope was used to count the survival neurons with neurites and measure the average length of neurites.MTT and LDH assay were carried out to investigate the effect of PCA on the neuronal viability.Results:Compared with control group,different concentration of PCA could increase t... |
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| Keywords: | Protocatechuic acid Cerebral cortex neuron Neurotrophic effects |
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