输血中采用微柱凝胶抗人球蛋白法检测不规则抗体的临床分析

目的分析在输血中采用微柱凝胶抗人球蛋白法检测不规则抗体的意义。方法针对近期需要输血的1983例病例（2010年4月-2012年3月）实施不规则抗体检测，所有病例均采用微柱凝胶抗人球蛋白法，统计阳性率并行抗体分析。结果5例病例的检测结果呈阳性，阳性率统计值0．25％（5／1983），2例（0．10％）表现为抗-E，1例（0．05％）表现为抗-cE，1例（0．05％）表现为抗-c，1例（0．05％）表现为抗-D。结论在输血中采用微柱凝胶抗人球蛋白法检测不规则抗体具有较高的灵敏性，能有效的减少溶血反应，提高输血安全性。

Irregular antibody detection by micro column gel Coombs test in blood transfusion

Objective To analyze the significance of irregular antibody detection by micro column gel Coombs test in blood transfusion. Methods According to the need of blood transfusion in 1 983 cases（Apr 2010-Mar 2012）implementation of irregular antibody detection, all the cases were treated by micro column gel Coombs test, statistical analysis of parallel antibody positive rate. Results The 5 cases＂ test resuhs were positive, the positive rate statistics was 0.25% （5/1983）,2 cases （0.10%） showed resistance to-E, 1 cases（0.05%） showed resistance to-cE, 1 cases（0.05%） showed resistance to-c and 1 cases （0.05%） showed resistance to-D. Conclusion The detection of irregular antibodies in blood transfusion by using micro column gel Coombs test has high sensitivity, reduce the occurrence of hemolytic reaction probability effectively and improve the safety of blood transfusion.