二甲基亚砜对CAOV3细胞端粒酶活性的可逆性抑制作用

目的探讨卵巢癌细胞CAOV3经二甲基亚砜（Dimethyl sulfoxide，DMSO）作用后及停止用药后端粒酶活性变化与细胞周期进展之间的关系。方法台盼蓝拒染法计数活细胞，流式细胞术观察细胞周期分布，端粒重复序列扩增法（TRAP）、聚丙烯酰胺凝胶电泳和银染法检测端粒酶活性。结果生长曲线显示DMSO对CAOV3细胞具有生长抑制作用，且具有浓度和时间依赖性。经1．4％DMSO作用96h后，CAOV3细胞主要受阻于G0／G1期，其端粒酶活性完全被抑制。去除药物后，细胞逐渐由G0／G1期向S期过渡，其端粒酶活性亦逐渐恢复，并于去除药物24h时，S期细胞百分率与端粒酶活性均明显高于用药前水平。结论DMSO对CAOV3细胞具有可逆性阻滞细胞周期进展，并下调其端粒酶活性的作用。CAOV3细胞端粒酶活性表达可能存在细胞周期依赖性调节。

Reversible inhibition of DMSO on telomerase activity of CAOV3 cells

Objective] Our aim was to investigate the relationship between the change of telomerase activity and the cell cycle in ovarian cancer cell CAOV3 line after exposure to and release from DMSO. Methods] Trypan blue exclusion was used to count the number of alive cells. The distribution of cell cycle was observed with flow cytometrie analysis. The activity of telomerase was examined by TRAP, polyacrylamide gel electrophoresis, and silver staining. Results] The growth curve shows that the proliferative ability of CAOV3 cells was gradually inhibited with raised concentrations and prolonged treatment of DMSO. CAOV3 cells were mainly arrested at G0/G1 phase, and their telomerase activity was down-regulated after exposure to 1.4% DMSO for 96 h. But releasing from DMSO, CAOV3 cells were gradually transmitted from G0/G1 phase to S phase, and the telomerase activity also recovered rapidly. After releasing from DMSO for 24 h, both the percentage of cells at S phase and the telomerase activity in CAOV3 cells were prominently higher than those in control groups. Conclusion] DMSO can cause a reversible G0/G1 phase arrest and inhibition of telomerase activity in CAOV3 cells. The change of telomerase activity may depend on the modulation of progression of cell cycle in CAOV3 cells.