糖尿病早期大鼠坐骨神经形态学的实验研究

目的：研究糖尿病早期大鼠周围神经形态学变化，为糖尿病周围神经病变动物模型的确立提供客观依据。方法：采用链脲佐菌素（STZ）诱导大鼠建立糖尿病模型（DM），光镜及电镜下观察大鼠6周末坐骨神经病理形态学变化，并对坐骨神经横切面进行定量分析。结果：DM大鼠6周时坐骨神经出现明显的病理损害。光镜下见坐骨神经有髓鞘神经纤维空泡样变性、轴索肿胀。电镜下见严重脱鞘改变。病理图像分析结果与电镜结果相符。结论：STZ诱导大鼠早期已出现明显的周围神经损害，符合糖尿病周围神经病变（DPN）的病理特点，说明DPN模型建立成功。

Study of the Peripheral Neuropathy in the Early Stage of Diabetic Rats

Objective:To study the peripheral nerve changes in diabetic rats in the early stage,in order to proffer an objective and theoretic reference for the establishment of animal model being diabetic peripheral neurophathy.Methods:The models of rats were induced by Strepotozotocin(STZ).After 6 weeks,the histomorphometry of sural nerve was analysed with light microscope and electron microscpe.Results: Morphometric analysis showed that the fibers density in the nerve of diabetic rat was significantly fewer than that of normal group(P <0.01).Conclusion:Peripheral neurophathy of STZ-induced diabetic rats appeared in the early stage,corresponded with the histomorphometry of diabetic peripheral neurophathy. It certifies that the model of diabetic peripheral neurophathy is successful.