反相高效液相色谱法测定微量血浆中卡马西平浓度

目的 :建立微量血浆中卡马西平浓度的测定方法。方法 :采用反相高效液相色谱 (HPLC)法 ,以艾司唑仑为内标 ,测定微量血浆中卡马西平浓度。色谱柱 :ShimadzuShimpackCLC -ODS 6 0mm× 15 0mm ,加保护柱 ,流动相为甲醇和水 (6 0∶40 ) ,流速 0 8ml/min ,检测波长为 2 5 4nm。结果 :卡马西平在 2～ 16mg/L浓度范围内线性良好 ,r=0 9996。最低检测限为 4 92 μg/L。卡马西平高、中、低 3种浓度的平均回收率分别为 97 0 0 % ,96 0 0 % ,93 98% ;RSD分别为 1 1% ,3 0 % ,4 1% (n =9)。日内RSD分别为 1 9% ,1 6 % ,6 3% (n =5 )。日间RSD分别为 1 8% ,2 4% ,2 9% (n =5 )。结论 :该方法准确、快速、简便 ,可作为卡马西平血药浓度常规监测方法

Determination of Carbamazepine Concentration in Plasma by RP-HPLC

To establish a method for rapid determination of Carbamazepine in human plasma.Methods: Using RP-HPLC with Estazolam as internal standard to determine concentration of Carbamazepine in human plasma. The separation was performed on shimadzu shimpack CLC-C18 column with methanol-water (60∶40) as mobile phase. The flow rate was 0.8 ml/min . The wavelength for detection was 254 nm. Results: The linear range of Carbamazepine was between 2～16 mg/L with r=0.999 6. The lowest limit of detection was 4.92 μg/L. The average recoveries for carbamazepine in three different concentrations were 97.00%，96.00%，93.98% with RSD of 1.1%, 3.0% and 4.1% respectively (n=9). The RSD values of intraday and interday were 1.9%，1.6%，6.3% (n= 5) and 1.8%, 2.4%, 2.9% ( n= 5 ). Conclusion: This method is accurate, rapid and easy. It can be used as a routine method in TDM.