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| 高效液相色谱法测定枳术颗粒中柚皮苷的含量 | |
| 引用本文： | 周骏,刘美辉,金顺福,濮存海. 高效液相色谱法测定枳术颗粒中柚皮苷的含量[J]. 中国药房, 2006, 17(14): 1103-1104 |
| 作者姓名： | 周骏 刘美辉 金顺福 濮存海 |
| 作者单位： | 1. 南京大学医学院附属鼓楼医院,南京市,210008 2. 延边大学医学院,延边市,133000 3. 南京中山制药厂,南京市,210012 |
| 摘 要： | 目的建立以高效液相色谱法测定枳术颗粒中柚皮苷含量的方法。方法色谱柱为Shim-packCLC-ODS,流动相为乙腈-水(20∶80,磷酸调节pH值至3.0±0.1),检测波长为283nm,流速为1.0ml/min,柱温为室温。结果柚皮苷进样量在0.1208μg~3.0200μg范围内线性关系良好(r=0.9999);平均加样回收率为99.1%(RSD=2.0%)。结论本方法简便、准确、灵敏度高、分离度与重现性好,可用于本品的含量测定与质量控制。 |
| 关 键 词： | 高效液相色谱法 枳术颗粒 柚皮苷 含量 |
| 文章编号： | 1001-0408(2006)14-1103-02 |
| 收稿时间： | 2006-01-01 |
| 修稿时间： | 2006-02-02 |
Content Determination of Naringin in Zhizhu Granules by HPLC |
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| ZHOU Jun,LIU Meihui,JIN Shunfu,PU Cunhai. Content Determination of Naringin in Zhizhu Granules by HPLC[J]. China Pharmacy, 2006, 17(14): 1103-1104 | |
| Authors: | ZHOU Jun LIU Meihui JIN Shunfu PU Cunhai |
| Abstract: | OBJECTIVE:To establish a method for the content determination of Naringin in Zhishu granules by HPLC. METHODS:The Shim-pack CLC-ODS column was used.The mobile phase consisted of acetonitrile-water(20∶80,adjusted to pH value of 3.0±0.1 with phosphoric acid).The detection wavelength was set at 283nm,the flow rate at 1.0ml/min,and the column temperature at room temperature.RESULTS: The calibration curve of Naringin was in good linearity over the range of 0.1 208μg～3.0 200μg(r=0.9 999).The average recovery was 99.1%(RSD=2.0%).CONCLUSION: The present method is simple,accurate,sensitive as well as with good resolving power and reproducibility,which can be used for the content determination and quality control of Zhishu granules. |
| Keywords: | HPLC Zhizhu granules Naringin Content |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |