145株非结核分枝杆菌对10种药物的耐药性分析

目的了解我院非结核病患者分离的非结核分枝杆菌对10种药物的耐药状况。方法用MB/Bact 240分枝杆菌培养仪和改良罗氏管对患者的多种标本进行分枝杆菌分离培养鉴定,对分离到的分枝杆菌采用绝对浓度法对10种抗结核药物,利福平、异烟肼、乙胺丁醇、链霉素,利福喷丁、丙硫异烟肼、氧氟沙星、卷曲霉素、卡那霉素和对氨基水扬酸进行药物敏感性试验。结果 1722例患者的标本非结核分枝杆菌培养阳性145株,对10种药总耐药率97.2%（141/145）,单耐药率最高为异烟肼、链霉素和对氨基水扬酸,最低为氧氟沙星。结论非结核分枝杆菌耐药情况十分严重,应加强抗结核药物的耐药性监测;根据药敏试验选择科学有效的化疗方案。

Analysis of drug resistance of 145 strains nontuberculous mycobacteria

Aim To analyze the drug resistance of nontuberculous mycobacteria（NTM） for 10 type antituberculosis drugs in Shenzhen Municipal Third People＇s Hospital.Methods All kinds of samples were cultured with MB/Bact 240 and improved L-J medium,clinical isolated mycobacteria strains were tested with absolute concentration method for 10 type antituberculosis drugs,including RFP、INH、EMB、SM、RFT、TH、OFL、CPM、KM and PAS.Results 145 strains NTM were isolated in 1722 samples,the total ratio of drug resistant for NTM for 10 type antituberculosis drugs is 97.2%（141/145）;Of 10 type antituberculosis drugs,the highest resistant rate is INH、SM and PAS,the lowest is OFL.Conclusions Clinical isolated NTM of Shenzhen area is very serious on drug resistant,monitoring of drugs resistance to NTM must be strengthened;Choosing reasonable and efficient therapy on guideline of antituberculosis susceptibility test.