重组骨形成发生蛋白-7促进种植体骨组织界面形成实验研究

目的：观察重组骨形成发生蛋白-7(BMP-7)对种植体骨组织界面形成的作用。方法：构建种植体植入动物模型，以正常侧作为对照，应用组织学方法观察种植体骨结合界面形态，组织化学方法测定碱性磷酸酶(ALP)活性，电子探针X射线能谱分析系统测定界面元素变化。结果：实验组在2周时，ALP活性较高，2至6周时迅速升高接近峰值，逐渐稳定直至12周。而对照组均匀升高，两组在各个时间段ALP活性差异都有统计学意义（P＜0.05）。组织学观察发现，2周时种植体骨界面就有新生骨组织形成，6周时种植体界面已形成大量新生骨组织，种植体与机体组织之间只有极其薄的一层纤维膜，已达到骨结合标准。电子探针X射线能谱分析表明，种植体植入后6周内，钙元素与磷元素逐渐增加并达到峰值，以后趋于平稳。而对照组在各项检测中都较实验组迟缓。结论：BMP-7可以加速骨种植界面的愈合。

Promotirg osteogenesis by recombined human BMP-7 at the interface of oral implants

Objective: To investigate the role of recombined human BMP-7 in the bone formation of implanted interface microregion.Methods: A histological microscope was used to observe the morphology,the histological chemistry method was used to determine the ALP activity and electronic probe X-ray microregion analysis was used to evaluate the element of the interface.Results: Two weeks after the implantation,an amount of new bone formation was observed through the histological microscope and at 6 weeks it nearly reached the bone integrated.The ALP activity was very high at two weeks after the implantation and almost reached a peak value at 6 weeks,and then it remained steady.The percentage of Ca and P increased rapidly and reached a peak at 6 weeks,and then it remained steady and persisted with the time.Conclusion: Recombination human BMP-7 may promote bone interface formation in oral implants.