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TAG-1与神经干细胞分化的相关性研究
引用本文:纳冬梅,胡继良,文艳红,杨振九,孙强,林文娟.TAG-1与神经干细胞分化的相关性研究[J].中国医药导报,2013,10(9):12-14.
作者姓名:纳冬梅  胡继良  文艳红  杨振九  孙强  林文娟
作者单位:纳冬梅 (广东省深圳市蛇口人民医院神经科,广东深圳,518000);胡继良 (广东省深圳市第六人民医院南山人民医院神经外科,广东深圳,518052);文艳红 (广东省深圳市蛇口人民医院神经科,广东深圳,518000);杨振九 (广东省深圳市第六人民医院南山人民医院神经外科,广东深圳,518052);孙强 (广东省深圳市第六人民医院南山人民医院神经外科,广东深圳,518052); 林文娟 (广东省深圳市第六人民医院南山人民医院神经外科,广东深圳,518052);
基金项目:广东省深圳市科技计划项目(项目编号:编号201002174)
摘    要:目的研究微环境蛋白TAG-1与神经干细胞分化之间的关系。方法使用免疫荧光染色检测神经干细胞中巢蛋白nestin的表达,并过表达TAG-1后使用实时荧光定量PCR技术分析神经干细胞中分化相关蛋白的表达变化。结果体外培养的神经干细胞中过表达TAG—1以后,干性相关基因nestin和转录因子SOX2(SRY—box2)表达降低,神经元标志物神经元微管蛋白β Ⅲ (neuronalclass Ⅲ β-Tubulin,Tujl)和胶质细胞标志物胶质纤维酸性蛋白(GFAP)表达升高。结论TAG-1可能与神经干细胞的分化存在正相关的关系,其分子调节机制有待进一步研究。

关 键 词:TAG-1  神经干细胞  分化

Research on TAG-1 and neural stem cell differentiation
NA Dongmei,HU Jiliang,WEN Yanhon,YANG Zhenjiu,SUN Qian,LIN Wenjuan.Research on TAG-1 and neural stem cell differentiation[J].China Medical Herald,2013,10(9):12-14.
Authors:NA Dongmei  HU Jiliang  WEN Yanhon  YANG Zhenjiu  SUN Qian  LIN Wenjuan
Institution:1.Department of Neurology, Shenzhen Shekou People's Hospital, Guangdong Province, Shenzhen 518000, China; 2.Department of Neurosurgery, the Sixth People's Hospital of Shenzhen City Nanshan People's Hospital, Guangdong Province, Shenzhen 518052, China
Abstract:Objective To investigate the association between TAG-1 expression and neural stem cells differentiation. Methods Immunofluorescence stain for nestin was performed to indentify NSCs cultured in vitro. Expression change of differentiation associated genes in mRNA level was detected by Real time PCR. Results After NSCs over expressed TAG-l, the mRNA level of nestin and SOX2 were decreased, Neuronal Class Ⅲ β-Tubulin (Tujl) and glial fibrillary acidic protein (GFAP) were upregulated. Conclusion TAG-1 may participate in the differentiation of neural stem cells, the molecular mechanism needs further research.
Keywords:TAG-1  Neural stem ceils  Differentiation
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