纳米羟基磷灰石/聚酰胺复合人工椎板预防脊柱后路术后椎管内瘢痕粘连的实验研究

目的：探讨纳米羟基磷灰百／聚酰胺（nano-hydmxyapatite／polyamide-66，n-HA／PA66）复合人工椎板在防止脊柱后路术后椎管内瘢痕粘连，重建脊柱后部结构完整性的价值。方法：22只1岁龄雄性家犬行L5椎板切除，实验组13只行复合人丁椎板植入，另9只作空白对照。术后4、8、16、24周各时相点摄X线片，行组织学、扫描电镜观察；术后24周行MRI检查．结果：实验组术后16～24周复合人工椎板界面观察到软骨细胞及板层骨，硬膜粘连程度比对照组明瞳减轻（P〈0．01）。术后24周时MRi显示实验组椎管完整性恢复。结论：n-HA／PA66复合材料具有良好的生物相容性，能有效阻止硬膜外粘连。

An experimental study of nano-hydroxyapatite/polyamide-66 composite material artificial vertebral lamina on preventing the postlaminectomy scar formation and the dura adherence

Objective:To evaluate the effect of artificial vertebral lamina made from nano-hydroxyapatite/polyamide-66(n-HA/PA66) on preventing the postlaminectomy scar formation and the dura adherence in the spinal canal,and constructing vertebral canal posterior elements integraty.Method:Of the 22 male dogs aged one year(13 in experimental group,9 in control group) the L5 laminectomy were ressected and the L5 lamina were reconstructed in the experimental group by implanting n-HA/PA66 composite material.The plain roentgenography,histology and scanning electron microscope were used at the 4th,8th,16th and 24th week respectively after the operation.MRI was undertaken at the 24th week.Result:At the 16th~24th week after the operation,the cartilage cell and lamellar bone were observed in the experimental group.Compared with the control group,the dura adhesion in the laminoplasty segment decreased significantly(P<0.01).The reconstructed segment retained the spinal integraty.Conclusion:n-HA/PA66 composite material not only has good biocompatibility,but also can effectively prevent the scar formation and dura adhesion.