强直性脊柱炎患者血清IL-12水平及临床意义研究

目的探讨血清IL-12p70含量在强直性脊柱炎（AS）和健康个体间的差异及其临床意义。方法应用EHSA方法检测60例AS患者和30例健康对照血清IL-12p70水平；应用ESR自动化分析仪和特定蛋白分析仪分别测定红细胞沉降率（ESR）和血浆C反应蛋白（CRP）；分析HLA-B27阳性和阴性的AS患者血清IL-12p70水平及与ESR、CRP的相关性。结果AS患者血清IL-12p70水平明显高于对照组（P〈0．01），且HLA-B27阳性和阴性AS患者均高于对照组（P〈0．01），两者比较，差异无显著性（P〉0．05）。在HLA-B27阳性AS患者中，血清IL-12p70水平与ESR及CRP均呈明显的相关性，但在HLA-B27阴性AS患者中则无显著相关性。结论AS患者血清IL-12p70水平明显上调，且这种上调与HLA-B27阳性与否无明显相关性。但AS患者血清IL-12p70水平与ESR及CRP是否呈显著相关竹却明显受到HLA-B27阳性与否的影响，进一步提示血清IL-12p70水平的改变可能与AS的发生和发展密切相关。

The study on serum level of IL-12 in patients with ankylosing spondylitis and its clinical significance

Objective To explore the difference of serum IL-12p70 level between the patients with ankylosing spondylitis(AS) and healthy individuals,as well as its clinical significance.Methods By ELISA,the concentration of serum IL-12p70 was measured in 60 AS patients and 30 healthy controls.Erythrocyte sedimentation rate and C-reactive protein were detected automatically respectively by ESR automatic analyzer and specific protein analyzer.The correlations between the level of serum IL-12p70 and ESR,CRP were analyzed in both HLA-B27 positive and negative AS patients.Results The level of serum IL-12p70 was significantly higher in AS patients than in the controls.Furthermore,both HLA-B27 positive and negative AS patients showed higher IL-12p70 level than control.However,no significant difference was found between these two groups.Among HLA-B27 positive AS patients,close correlations between the level of serum IL-12p70 and ESR,CRP were observed,but no correlation was observed in HLA-B27 negative patients.Conclusion The concentration of serum IL-12p70 was elevated in AS patients,which was not associated with whether HLA-B27 was positive or not.However,the correlation between the level of serum IL-12p70 and ESR,CRP was clearly influenced by whether HLA-B27 positive or not in AS patients.The results above indicated that the altered level of serum IL-12p70 might show a close association with the development and progression of AS.