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住院患者耐甲氧西林金黄色葡萄球菌定植与感染相关研究
引用本文:刘帅,韩雪琳,尹丽霞,李帅,张玉梅,胡小华,于仁涛,王菡,李云,乔媛媛,岳启安,韩黎. 住院患者耐甲氧西林金黄色葡萄球菌定植与感染相关研究[J]. 中国感染控制杂志, 2008, 7(5)
作者姓名:刘帅  韩雪琳  尹丽霞  李帅  张玉梅  胡小华  于仁涛  王菡  李云  乔媛媛  岳启安  韩黎
作者单位:1. 潍坊医学院医学微生物学教研室,山东,潍坊,261042
2. 解放军疾病预防控制所医院感染监督中心,北京,100071
摘    要:目的调查住院患者耐甲氧西林金黄色葡萄球菌(MRSA)定植及感染的状况,分析MRSA定植与感染的关系。方法对某大医院神经内科住院患者在入院48h内及以后每周一、三、五采集鼻前庭标本,同时收集该期间住院患者送检临床标本中分离的MRSA菌株。以低频限制性位点聚合酶链反应(IRS—PCR)基因分型法对患者鼻前庭标本和临床标本分离的MRSA菌株进行同源性分析。结果共调查265例患者,MRSA定植者14例(5.28%),其中7例为入院前定植,7例为入院后定植;MRSA感染者10例(3.77%),其中8例的鼻前庭标本和临床标本均分离出MRSA,另2例仅临床标本分离出MRSA。IRS—PCR基因分型显示MRSA感染患者临床标本和鼻前庭标本分离出的MRSA具有很高的同源性。结论住院患者定植MRSA是发生MRSA医院感染的一个重要危险因素。

关 键 词:金黄色葡萄球菌  耐甲氧西林金黄色葡萄球菌  定植  医院感染  基因分型  同源性  IRS—PCR

Study on methicillin-resistant Staphylococcus aureus colonization and infection among inpatients
LIU Shuai,HAN Xue-lin,YIN Li-xia,LI Shuai,ZHANG Yu-mei,HU Xiao-hua,YU Ren-tao,WANG Han,LI Yun,QIAO Yuan-yuan,YUE Qian,HAN Li. Study on methicillin-resistant Staphylococcus aureus colonization and infection among inpatients[J]. Chinese Journal of Infection Control, 2008, 7(5)
Authors:LIU Shuai  HAN Xue-lin  YIN Li-xia  LI Shuai  ZHANG Yu-mei  HU Xiao-hua  YU Ren-tao  WANG Han  LI Yun  QIAO Yuan-yuan  YUE Qian  HAN Li
Affiliation:LIU Shuai1,HAN Xue-lin2,YIN Li-xia2,LI Shuai2,ZHANG Yu-mei2,HU Xiao-hua2,YU Ren-tao2,WANG Han2,LI Yun1,QIAO Yuan-yuan2,YUE Qi-an1,HAN Li2(1.Department of Medical Microbiology,Weifang Medical College,Weifang 261042,China,2.Center for Hospital Infection Supervision,Institute of Disease Control , Prevention of PLA,Beijing 100071,China)
Abstract:Objective To investigate the prevalence of methicillin-resistant Staphylococcus aureus(MRSA) colonization and infection among inpatients,and the relation between MRSA colonization and infection.Methods Specimens of nasal vestibule from all patients in neurology department at admission and every Monday,Wednesday,Friday thereafter were taken and performed bacterial culture,MRSA strains isolated from the nasal vestibule and clinical specimens were collected.The genes of the isolated MRSA strains were amplified...
Keywords:IRS-PCR
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