| 一氧化氮对大鼠主动脉血管平滑肌细胞骨桥蛋白表达的影响 |
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| 引用本文： | 张瑶,郭文怡,王臻,王海昌,李寰,陶凌,栾荣华,胡涛. 一氧化氮对大鼠主动脉血管平滑肌细胞骨桥蛋白表达的影响[J]. 临床心血管病杂志, 2009, 25(9) |
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| 作者姓名： | 张瑶 郭文怡 王臻 王海昌 李寰 陶凌 栾荣华 胡涛 |
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| 作者单位： | 1. 第四军医大学西京医院骨科,西安,710032
2. 第四军医大学西京医院心内科 |
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| 摘 要： | 目的:观察一氧化氮(NO)对培养的大鼠主动脉血管平滑肌细胞(VSMC)骨桥蛋白(OPN)表达的影响。方法:体外培养大鼠主动脉VSMC,随机分为对照组,不同浓度NO供体S-亚硝基-N-乙酰青霉胺(SNAP,0.5,1,2,5mmol)干预组,应用RT-PCR及Western blot技术结合光密度扫描分析,观察SNAP对VSMC的OPN表达的影响。结果:不同浓度SNAP均明显抑制VSMC的OPN mRNA和蛋白的表达,且具有剂量依赖性抑制作用。结论:NO能抑制VSMC的OPN表达。
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| 关 键 词： | 血管平滑肌细胞 一氧化氮 骨桥蛋白 基因表达 蛋白表达 |
Nitric oxide inhibits osteopontin expression in rat aortic vascular smooth muscle cells |
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| ZHANG Yao GUO Wenyi WANG Zhen WANG HaichangLI Huan TAO Ling LUAN Ronghua HU Tao. Nitric oxide inhibits osteopontin expression in rat aortic vascular smooth muscle cells[J]. Journal of Clinical Cardiology, 2009, 25(9) |
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| Authors: | ZHANG Yao GUO Wenyi WANG Zhen WANG HaichangLI Huan TAO Ling LUAN Ronghua HU Tao |
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| Affiliation: | 1Department of Orthopaedics;2Department of Cardiology;Xijing Hospital;Fourth Military Medical University;Xi'an;710032;China |
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| Abstract: | Objective:To investigate the effect of nitric oxide (NO) on osteopontin expression in rat aortic vascular smooth muscle cell(VSMC).Method:Rat aortic VSMC were cultured in vitro and incubated with different concentration of S-nitroso-N-acetylpenicillamine(SNAP),a nitric oxide donor for 48 hours. Then RT-PCR and Western bloting analysis were used to observe the osteopontin expression in rat VSMC.Result:Different concentration of SNAP could obviously reduce the mRNA expression and protein synthesis of OPN with... |
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| Keywords: | smooth muscle cells nitric oxide osteopontin gene expression protein synthesis |
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