西藏地区几株虫媒病毒的分离与初步鉴定

对西藏亚西和之那地区采集的蜱，鸟，鼠和家畜血标本用ＢＨＫ１３细胞和１－３日龄乳小白鼠进行了病毒分离，共获得了１７株病毒，对其中两株（Ｔ２０６６，Ｔ２０７６）病毒进行了初步鉴定，包括电镜形态观察，理化，生物学和免疫学性质试验。在９种已知的虫媒病毒及其标准免疫血清（ＳＩＮ，ＣＨＩＫ，ＭＡＫ，ＥＥＥ，ＭＶＥ，ＪＢＥ，ＴＢＥ，ＹＦ－１７Ｄ，ＨＦＲＳ）和分离毒株的交互间接免疫荧光试验中，未发现血清学反应，用

The Viruses Isolated from the Specimehs Collected in the Tibet and Primary Identification

Yoxi and Zhila of the Tibet in the Southwest border line of China,the ecological factors of the localities sustain abundant populations of vertebrates and arthropods for shelter,development and survival. A great quantity of specimens of ticks,mice and birds from these spaces were collected. And 17 strain viruses came from the specimens,3 of them from the ticks, 1 strain from the birds and 13 strains from the mice. Primary identification for 2 strain viruses (No. 2066 from the birds and No. 2076 from the mice)among them have been made. The replication occurred in BHK-13 cells with CPE in 4 day. The mice infected with the viruses that affected the central nervous system and/or followed by death. The 2 strain viruses were examined by ultrathin sec-tion and negative contrast electron microscopy. The results of serologicol identification showed that the 2 strain viruses were not any antigenically related to 9 known arboviruses (SIN,CHIK, MAY,EEE,MVE,JBE,TBE,YF-17D,HFRS) by IF AT. And 188 sera samples of the local peo ple were detected by IF AT. The results showed that there were the 2 strain viruses antibodies in the sera samples. The positive rate were 26% (49/188 ). According to the studies mentioned above,it indicated that the isolated 2 strain viruses belonged to Reoviridae. So far as we know it may belong to arbovirus.