实验性自身免疫性葡萄膜炎T淋巴细胞亚群与Treg细胞的表达研究

目的　探讨实验性自身免疫性葡萄膜炎（ｅｘｐｅｒｉｍｅｎｔａｌａｕｔｏｉｍｍｕｎｅｕｖｅｉｔｉｓ，ＥＡＵ）新西兰兔模型外周血单个核细胞（ｐｅ-ｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕｃｌｅａｒｃｅｌｌｓ，ＰＢＭＣ）Ｔ淋巴细胞亚群的变化及其与疾病特征的相关性。方法　选取健康成年雄性新西兰大白兔４０只作为动物模型，用２０ｇ·Ｌ－１牛血清蛋白（ｂｏｖｉｎｅｓｅｒｕｍａｌｂｕｍｉｎ，ＢＳＡ）静脉注射和玻璃体内注射法建立ＥＡＵ模型，并观察模型眼炎症反应及ＰＢＭＣＴ淋巴细胞亚群特征。结果　ＣＤ４＋Ｔ细胞在ＥＡＵ兔的外周血中比例明显增加，且随时间的进展呈进行性增加，并与炎症反应呈正相关，相同的趋势可见于ＣＤ４＋／ＣＤ８＋Ｔ细胞比例。此外，Ｔｒｅｇ细胞趋势与ＣＤ４＋Ｔ细胞及ＣＤ４＋Ｔ／ＣＤ８＋Ｔ细胞比例相反，与炎症反应呈负相关。结论　ＣＤ４＋Ｔ细胞的优势性选择性克隆增殖及Ｔｒｅｇ细胞的降低有可能引发免疫功能紊乱并导致针对自身组织的免疫应答失去有效的负性调控，导致ＥＡＵ的发生及进行性加重。

Changes of T-cell subpopulation and T regulatory cells in experimental autoimmune uveitis

Objective To investigate the changes of T-cell subpopulation in the peripheral blood mononuclear cells ( PBMC) and it ’ s association with the model of experimental autoimmune uveitis ( EAU) in New Zealand rabbits. Methods EAU models in 40 healthy male adult New Zealand rabbits were established by intravenous or rntravitreal injection of 20 g . L -l bovine serum albunun . and the inflammation in the operated eyes and the changes of T cells subpopulation in the PBMC were observed. Results The percentage of CD4 + T cells increased progressively in the PBMC of EAU group over time ,which was positive correlated with the inflammation. Besides , the tendency of the ratio of CD4 + /CD8 + T cells was the same as above. While ,the tendency of T regulatory cells ( Treg cells) was opposite as above ,negatively correlated with the inflammation. Conclusion The donunant selection of CD4 + T cells clonal expansion and reduction of Treg cells may lead to immunity functional disturbance . and lose efficiently negative regulation of autoimmune response ,which will both result in and aggravate EAU.