紫外光A/核黄素角膜交联后兔角膜基质内基质金属蛋白酶-1含量的变化

目的　检测紫外光Ａ／核黄素角膜交联（ｕｌｔｒａｖｉｏｌｅｔＡ／ｒｉｂｏｆｌａｖｉｎｃｏｒｎｅａｌｃｒｏｓｓ-ｌｉｎｋ-ｉｎｇ，ＵＶＡＲＣＸＬ）后兔角膜基质内基质金属蛋白酶（ｍａｔｒｉｘｍｅｔａｌｌｏｐｒｏｔｅｉｎａｓｅ，ＭＭＰ）-１的短期含量变化。方法　１５只新西兰白兔，随机分为３组，每组５只，Ａ组为空白对照组，Ｂ组为角膜交联后３ｄ，Ｃ组为角膜交联后７ｄ。Ｂ、Ｃ两组行ＵＶＡＲＣＸＬ。各组取角膜后去除角膜上皮和内皮细胞，应用ＥＬＩＳＡ法检测角膜基质内ＭＭＰ-１的含量。结果　Ａ组角膜基质内ＭＭＰ-１／总蛋白含量为（０．１４０±０．０３６）×１０－６，Ｂ组为（０．２４２±０．０５９）×１０－６，Ｃ组为（０．３７２±０．０６１）×１０－６，３组之间差异有统计学意义（Ｆ＝２４．０５１，Ｐ＝０．０００）。ＵＶＡＲＣＸＬ后３ｄ，兔角膜基质内ＭＭＰ-１含量显著升高，与Ａ组相比差异有统计学意义（Ｐ＜０．０５），术后７ｄ其含量进一步升高，与Ｂ组相比差异有统计学意义（Ｐ＜０．０１）。结论　ＵＶＡＲＣＸＬ后７ｄ内，兔角膜基质内ＭＭＰ-１含量持续增加，推测ＭＭＰ-１可能对ＵＶＡＲＣＸＬ后的角膜基质重塑起一定作用。

Changes of MMP-1 in rabbit corneal stroma after ultrovialet A/riboflavin corneal cross-linking

Objective To determine the content changes of matrix metalloproteinase-l ( MMP-I ) in rabbit corneal stroma in the short term after ultrovialet A/ riboflavin corneal cross-linking ( UVARCXL) . Methods Fifteen New Zealand white rabbits were randomLy divided into three groups, 5 rabbits in each group. Group A : Control group ; Group B:3 days after UVARCXL; Group C:7 days after UVARCXL. MMP-I content in corneal stroma was measured by ELISA after removing the corneal epithelium and endothelium. Results The proportion of MMP-I relative to the total protein of group A was (0. 140 + 0. 036) x 10 -6 , group B was (0. 242 + 0. 059 ) x 10 -C’ , and group C was (0. 372 +0. 061) x 10 -6 The difference among 3 groups was statistically sigruficant( F = 24. 05 1 .P = 0. 000 ) . Three days after UVARCXL . MMP-I content increased sigruficantly , and the difference was statistically significant compared with group A( P < 0. 05 ) . Seven days after UVARCXL. the content of MMP-I further increased. and the difference was statistically significant compared with group B ( P < 0. 01 ) . Coriclusion The content of MMP-I in rabbit corneal stroma increase continuously in 7 days after UVARCXL. which means that MMP-I may play a role in remolding corneal stroma after UVARCXL.