不同浓度KH902对兔碱烧伤后角膜新生血管的抑制作用

目的　观察不同浓度ＫＨ９０２对兔碱烧伤后角膜新生血管（ｃｏｒｎｅａｌｎｅｏｖａｓｃｕｌａｒｉｚａｔｉｏｎ，ＣＮＶ）的抑制作用。方法　取成年新西兰兔２４只，建立兔碱烧伤模型，将兔随机分为４组，使用ＫＨ９０２眼用注射剂（康柏西普）配置不同浓度的ＫＨ９０２，Ａ组２．５ｍｇ·ｍＬ－１，Ｂ组５ｍｇ·ｍＬ－１，Ｃ组１０ｍｇ·ｍＬ－１，Ｄ组为对照组（生理盐水），均为每日滴眼３次。分别在制模后３ｄ、７ｄ、１４ｄ、３０ｄ拍摄眼前节照片，观察ＣＮＶ情况并行角膜激光共焦显微镜检查，并计算ＣＮＶ面积。观察各组处死兔常规病理和ＶＥＧＦ表达情况。结果　在制模后２８ｄ内，四组ＣＮＶ形成，其中Ｄ组ＣＮＶ较Ａ、Ｂ、Ｃ组生长明显；对各组切片进行观察发现，四组均出现了角膜上皮及基质细胞的损失和修复、炎性细胞的浸润和ＣＮＶ的生长，但Ｄ组的炎性细胞较密集，ＣＮＶ管径和密度较高。制模后１４ｄ、２８ｄ，Ａ、Ｂ、Ｃ组炎性细胞密度较Ｄ组均显著减少（均为Ｐ＜０．０１）；在制模后７ｄ、１４ｄ、２８ｄ，Ａ、Ｂ、Ｃ组较Ｄ组ＶＥＧＦ表达明显减少；Ａ、Ｂ、Ｃ三组进行组间比较，仅制模后２８ｄ时Ｂ、Ｃ组间差异均无统计学意义（Ｐ＞０．０５），其余差异均有统计学意义（均为Ｐ＜０．０１）；制模后７ｄ、１４ｄ、２８ｄ，Ａ、Ｂ、Ｃ三组均较Ｄ组ＣＮＶ面积减少，差异均有统计学意义（均为Ｐ＜０．０１），而三组组间差异无统计学意义（Ｐ＞０．０５）。结论　不同浓度的ＫＨ９０２对兔碱烧伤后的ＣＮＶ均有抑制作用，低浓度的ＫＨ９０２即可产生明显的抑制作用。

Inhibitive effects of different concentrations of KH902 eye drops on corneal neovascularization after rabbit corneal alkali burn

Objective To investigate the inhibitive effects of different concentrations of KH902 eye drops on corneal neovascularization ( CNV) after rabbit corneal alkali burn. Methods Twenty-four adult rabbits were randomly divided into four groups , alkali bum model was established and divided into four groups, group A was treated with 2. 5 mg . mL-] KH902 eye drops, group B with 5 mg . mL-l KH902 eye drops , group C with 10 mg . mL -l KH902 eye drops , and control group with saline solution.3 times per day. At postoperative 3 days ,7 days ,14 days ,28 days , anterior segment photos were captured, the area of CNV was calculated, the confocal microscopy and pathological exanunation were given, and the vascular endothelial growth factor (VEGF) was analyzed quantitatively. Results CNV could be seen in four groups within 28 days , especially in group D from anterior segment photos. HE staining showed that the corneal epithelial and stromal damage and repair ,inflammatory cell infiltration and CNV grow were seen in four groups , but the inflammatory cells were more in group D , and the cube diameter and density of CNV were higher. At postoperative 14 days ,28 days ,the inflammatory cell density in group A, B , C were obviously lower than that in group D ( all P < 0. 01 ) . At postoperative 7 days , 14 days ,28 days , VEGF concentration in group A, B , C were obviously lower than that in group D ( all P < 0. 01 ) ; There were significant differences among group A, B, C ( all P < 0. 01 ) , except for 28 days between group B and C (P > 0. 05 ) . At postoperative 7 days, 14 days , 28 days , the CNV area in group A,B,C were obviously lower than that in group D ( all P < 0. 01) , and there was no statistical difference among group A, B, C ( P > 0. 05 ) . Conclusion KH902 eye drops with different concentrations have inhibitive effects on CNV after rabbit corneal alkali burn . even with lower concentration.