卵子玻璃化冷冻复苏后行卵母细胞胞浆内单精子显微注射27例临床结局

目的：探讨卵子玻璃化冷冻技术在辅助生殖中的临床应用价值。方法回顾性分析2013年1月－2015年12月在解放军174医院生殖医学中心行体外受精，取卵日因男方因素未取到精子而行卵子玻璃化冷冻保存的27对不孕夫妇的资料。卵子解冻后均行夫精卵母细胞胞浆内单精子显微注射，记录卵子受精情况及临床结局。结果27例患者共冷冻卵子245枚，并全部解冻，解冻后存活214枚，存活率84．79％；其中第二次减数分裂中期（ metaphase Ⅱ，MⅡ）卵198枚，并全部行ICSI，受精138枚，受精率74．49％；卵裂99枚，卵裂率71．74％；形成87枚2PN（原核，pronucleus）胚胎，优质胚胎34枚，优质胚胎率39?08％。20个移植周期移植胚胎41枚，临床妊娠6例，临床妊娠率34．82％。结论玻璃化冷冻技术应用于卵子冷冻保存，并对复苏卵子进行卵母细胞胞浆内单精子显微注射可以取得一定的临床妊娠率，在辅助生殖技术临床上具有很强的实用价值。

Clinical outcome of 27 intracytoplasmic sperm injection cycles with total oocytes cryopreserved using vitrifi-cation

Objective To investigate the clinical application value of vitrification cryopreservation of egg in human assistant reproduction. Methods A retrospective study was performed on patients underwent IVF/ICSI treatment in the reproductive medicine center in 174 hospital of PLA from 2013 to 2015. 27 couples with infertility without sperm for fertilization or insemination were includ?ed. All oocytes were cryopreserved using vitrificaiton and carried ICSI after oocyte were thawed. Basic characteristics, oocytes fertiliza?tion and clinical outcomes were analyzed. Results Totally 245 oocytes were cryopreserved using vitrification, and 214 oocytes sur?vived. The survival rate was 84. 79%; 198 mature oocytes performed ICSI treatment, and 138 oocytes fertilized. The fertilization rate was 74.49%;99 embryo cleaved after ICSI, and the cleavage rate was 71.74%;87 2PN embryos formed, and 34 embryos were high?quality embryos. The high?quality embryo rate was 39.08%. 20 cycles performed fresh embryo transfer, and 6 embryos got clinical preg?nancy. The clinical pregnancy rate was 34.82%. Conclusion A certain clinical pregnancy rate can be achieved in ICSI cycles with to?tal oocytes cryopreserved using vitrificaiton, and oocyte vitrification is practically useful in human assistant reproduction.