血卟啉衍生物光动力作用杀伤人胰腺癌细胞的实验研究

目的 探讨光动力作用（ＰＤＴ）对体外培养的人胰腺癌细胞的杀伤效应。方法 以２株人胰腺癌细胞系Ｐ３和ＳＷ１９９０为研究对象,采用血卟啉衍生物（ＨＰＤ）作为光敏剂,用高压钠灯（功率密度２５ｍＷ／ｃｍ＾２）为光源,以系列深度的ＨＰＤ经不同剂量的光照后,用ＭＴＴ法测定ＰＤＴ对胰腺癌细胞的相对抑制率。结果 随光敏剂浓度的升高和照光剂的增加,光动力作用对细胞的相对抑制率逐渐增大,在低光动力剂量下明显上升,随后

Photodynamic therapy of hematoporphyrin derivative on human pancreatic carcinoma cell lines

Objective To observe the effect of photodynamic therapy(PDT)on human pancreatic carcinoma cell lines in vitro. Methods Two lines of human pancreatic carcinoma cell P3 and SW1990 were studied to investigate the killing role of PDT with hematoporphyrin derivative(HPD) as photosensitizer and sodium lamp as light source. The cultured cells were treated with serial concentrations of HPD followed by irradiation of different dosages of visible light, then MTT colorometric assay was applied to measure the relative inhibitory rate of PDT for the cells. Results The relative inhibitory rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of light. Under the low photodynamic dose, it increased rapidly, and gradually slowed down to reach the plateau. Under the same photodynamic dose, significant difference in the inhibitory rate between the two lines was observed (P<0 01), and the LD90 was also different. Conclusions PDT has a significant killing effect on human pancreatic carcinoma cell lines in vitro, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and light irradiation. The sensitivity of the two cell lines to PDT is different.