Purification of neonatal rat cardiac cells by centrifugal elutriation |
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| Authors: | Roger G. Ulrich Kathryn A. Elliget Diane K. Rosnick |
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| Affiliation: | (1) Pathology and Toxicology Research, The Upjohn Company, 49001 Kalamazoo, Michigan;(2) Department of Pathology, University of Maryland, 21201 Baltimore, Maryland |
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| Abstract: | Summary A method utilizing centrifugal elutriation to obtain a highly purified (95 ± 2% after 3 d in culture) preparation of beating cardiac myocytes from enzyme-dissociated neonatal rat heart is described. An additional fraction enriched for nonmuscle cells is also obtained. Purity of myocyte cultures is determined by Coomassie blue R250 staining of the cytoskeleton; this procedure is also described. |
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| Keywords: | heart cell cardiac myocyte primary culture trypsin dissociation centrifugal elutriation |
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