乙型肝炎病毒中国株全基因参照序列的初步建立

目的初步建立乙型肝炎病毒中国株全基因参照序列。方法测定７例慢性无症状ＨＢＶ携带者ＨＢＶ基因组全序列，结合已有的２株ＨＢＶ中国株全基因序列，经同源性比较及对齐比较，确定基因型和参照序列。结果血清型ａｄｒ４例，ａｄｗ３例；２例基因型为Ｂ型，余均为Ｃ型。各序列间Ｘ基因差异最大。该参照序列与国外参照序列仅有２２个位点的差异。结论拟定了中国ＨＢＶＣ基因型毒株全基因参照序列，建立不同地区的ＨＢＶ毒株参照序列尚待进一步收集各地区ＨＢＶ全基因序列。

Establishment of the consensus sequence of hepatitis B virus prevailing in the mainland of China

Objective For producing the most possible consensus sequence of hepatitis B virus prevailing in the mainland of China. Methods Sequencing of the whole gene sequences of hepatitis B virus from seven Chinese asymptomatic carriers of hepatitis B virus was done, and consulting the 2 reported complete sequences of HBV, the nine whole sequences were compared with each other to determine the genotype and produce the consensus sequence. Results Two strains were of genotype B and the others were of genotype C, with serotype adr in 4 strains and adw in 3 strains. X gene region had most diverse among 9 strains of HBV. The consensus sequences produced from complete sequences of HBV prevailing in the mainland of China had 22 sites different from the consensus sequence from GenBank. Conclusions First time informally establishment of the consensus sequence of hepatitis B virus prevailing in the mainland of China. Further work is needed to do more whole gene sequencing of HBV strains from different regions of the mainland of China.