HLA多态性在广东汉族人群分布的特殊性

目的探讨人类白细胞抗原（ｈｕｍａｎｌｅｕｃｏｃｙｔｅａｎｔｉｇｅｎＨＬＡ）在广东汉族群体中的遗传特征。方法采用免疫磁珠单抗血清学技术进行ＨＬＡ－Ａ、Ｂ分型和聚合酶联反应－序列特异性引物（ｓｅｑｕｅｎｃｅｓｐｅｃｉｆｉｃｐｒｉｍｅｒｓＰＣＲ－ＳＳＰ）进行ＨＬＡ－ＤＲ、ＤＱ分型，调查了４０６名广东汉族健康献血员。结果识别ＨＬＡ－Ａ、Ｂ、ＤＲ和ＤＱ座位１０６个特异性，４１４２条单倍型，发现ＨＬＡ－Ａ３３－Ｂ５８－ＤＲ１７－ＤＱ２和ＨＬＡ－Ａ２－Ｂ４６－ＤＲ９－ＤＱ９在广东汉族中呈现高频率。结论ＨＬＡ－Ａ３３－Ｂ５８－ＤＲ１７－ＤＱ２和ＨＬＡ－Ａ２－Ｂ４６－ＤＲ９－ＤＱ９，这两条单倍型在广东汉族人群中的分布频率与相关文献其他民族和人群相比为显著连锁不平衡单倍型。

Characterization and distribution of HLA polymorphism in the Guangdong Han population

Objective To study the HLA genetic characters in the Guangdong Han population. Methods HLA A, B serological typing were performed with fluorobeads and monoclonal antibodies Terasaki typing trays. HLA DR, DQ DNA typing had analyzed by polymerase chain reaction with sequence specific primer (PCR SSP). Results Total 106 specificities were detected in HLA A, B DR, DQ, and 4 142 haplotypes were observed in 406 blood donors of the Guangdong Hans. The most frequently observed haplotypes were HLA A33 B58 DR17 DQ2 and HLA A2 B46 DR9 DQ9. Conclusions The haplotypes HLA A33 B58 DR17 DQ2 and HLA A2 B46 DR9 DQ9 were the most very strong linkage disequilibrium haplotype in the Guangdong Han population compared to those reported for other populations.