| 载脂蛋白A-I多态性研究 |
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| 引用本文： | 林荫,周国英,庄庆祺,梅美珍. 载脂蛋白A-I多态性研究[J]. 复旦学报(医学版), 1990, 0(1) |
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| 作者姓名： | 林荫 周国英 庄庆祺 梅美珍 |
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| 作者单位： | 上海医科大学药学院生物化学教研室(林荫，周国英，庄庆祺)，上海医科大学药学院生物化学教研室(梅美珍) |
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| 摘 要： | 用DEAE-52离子交换层析分离到载脂蛋白的A-I(apo A-I)的多态性。方法简便快速,制备量大。SDS-PAGE证实为纯apoA-I,分子量约28000。此多态性中apoA-I_1apoA-I_2、apoA-I_3是卵磷脂-胆固醇酰基转移酶(LCAT)的激活剂,apoA-I4则是LCAT的抑制剂。对其中的apoA-I_2进行氨基酸组成分析,发现不含半胱和异亮氨酸。apoA-I多态性的免疫电泳结果表明,各多态性与apoA-I_2抗体有免疫沉淀反应,说明多态性抗原部位相同。
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| 关 键 词： | 载脂蛋白A-I 多态性 卵磷脂-胆固醇酰基转移酶 |
STUDIES ON APOLIPOPROTEIN A-I POLYMORPHS |
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| Lin Yin,Zhou Guoying,Zhuang Qingqi,Mei Meizhen. STUDIES ON APOLIPOPROTEIN A-I POLYMORPHS[J]. Fudan University Journal of Medical Sciences, 1990, 0(1) |
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| Authors: | Lin Yin Zhou Guoying Zhuang Qingqi Mei Meizhen |
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| Abstract: | Polymorphs of apolipoprotein A-I(apoA-I) were isolated by DEAE-52 ion-exchange chromatography. This is a simple, rapid method and a large amounts of polymorphs were obtained by this method. The polymorphs were shown to be pure, with molecular weights 28 000, when analyzing with SDS-PAGE. The polymorphs, apoA-I1, apoA-I2, apoA-I3, were activators of lecithin: Cholesterol aoyltrangferase (LCAT) and apoA-I4 wag inhibitor of LCAT.It was found by amino acid composition analysis that apoA-I2, the major polymorph, did not contain oystelne or isoleuoine. Results of immunoalectrophoresia of apoA-I polymorphs showed that they have an immunoprecipitable reaction with the apoA-I2 antibody, indicating that all of the polymorphs have same antigenio site. |
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| Keywords: | apolipoprotein A-I polymorphism lecithin-cholesterol acyl- transferase |
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