三氧化二砷对RAJI细胞的诱导凋亡作用及其作用机制

目的：探讨三氧化二砷对白血病RAJI细胞的诱导凋亡作用及其作用机制。方法：以不同浓度的三氧化二砷作用于体外培养的RAJI细胞，MTT法检测细胞生长抑制率，应用流式细胞仪及瑞氏染色法观察细胞凋亡，TRAP—PCR-ELISA法检测细胞凋亡前后的端粒酶活性。结果：三氧化二砷可显著地降低RAJI细胞端粒酶活性，抑制细胞的生长，诱导细胞发生凋亡。并呈现出明显的量-效与时-效关系。结论：三氧化二砷能抑制RAJI细胞的生长并诱导细胞发生凋亡，降低细胞端粒酶活性可能是其重要作用机制之一。

Apoptotic effect of arsenic trioxide on RAJI cells and its mechanism

Objective To investigate the apoptotic effect of arsenic trioxide on leukemic RAJI cells and its mechanism. Methods RAJI cells in culture medium in vitro were given different concentrations of Arsenic trioxide.The inhibitory ratio of the cells were measured by MTT assay, apoptosis was obsevered by flow cytometry(FCM) and Wright staining, and the activity of telomerase was detected using TRAP PCR ELISA before and after apoptosis occurred. Results Arsenic trioxide could decrease the telomerase activity, cause apoptosis and inhibit the growth of RAJI cells significantly. The suppression was both in time and dose dependent form. Conclusion Arsenic trioxide can inhibit the growth of RAJI cells and induce apoptosis. Decreasing the telomerase activity of RAJI cells may be one of the most important mechamisms.