| 基于CdTe量子点和溴化乙锭-DNA共振能量转移的核酸检测 |
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| 引用本文： | 陈泽琴,印洁红,谢洪平.基于CdTe量子点和溴化乙锭-DNA共振能量转移的核酸检测[J].抗感染药学,2011,8(3):167-170. |
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| 作者姓名： | 陈泽琴 印洁红 谢洪平 |
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| 作者单位： | 1. 苏州大学医学部药学院,江苏苏州215123;西华师范大学化学化工学院,四川南充637002
2. 苏州大学医学部药学院,江苏苏州,215123 |
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| 基金项目： | 国家自然科学基金项目(No.81001686) |
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| 摘 要： | 目的:基于CdTe量子点和溴化乙锭-DNA共振能量转移的核酸定量检测。方法:通过EDC交联法制备了量子点-核酸探针,该探针与溴化乙锭-DNA通过DNA的互补配对形成荧光共振能量转移模型,借助这个模型进行核酸的定量检测。结果:该法对核酸目标链检测的线性范围为0.025-0.188μmol/L,其相关系数为0.997,最低定量限为0.025μmol/L。结论:基于CdTe量子点和溴化乙锭-DNA共振能量转移模型可以用于DNA的定量检测。
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| 关 键 词： | 核酸 量子点 溴化乙锭 荧光共振能量转移 |
Quantitative Detection of Nucleic Acid Based on Fluorescence Resonance Energy Transfer System between CdTe Quantum Dots and EB-DNA |
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| CHEN Ze-qin,YIN Jie-hong,XIE Hong-ping.Quantitative Detection of Nucleic Acid Based on Fluorescence Resonance Energy Transfer System between CdTe Quantum Dots and EB-DNA[J].Anti-infection Pharmacy,2011,8(3):167-170. |
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| Authors: | CHEN Ze-qin YIN Jie-hong XIE Hong-ping |
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| Institution: | CHEN Ze-qin1,2*2,YIN Jie-hong1,XIE Hong-ping1*3 1School of Pharmacy,Soochow University,Suzhou Jiangsu 215123,China,2School of Chemistry and Chemical Engineering,China West Normal University,Nangchong 637002 |
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| Abstract: | Objective ： To quantitatively detect nucleic acid based on the fluorescence resonance energy transfer system between the CdTe quantum dots and ethidium bromide-DNA. Methods： Quantum dots-DNA probe （CdTe-P） was obtained by using the EDC cross-linking method. Moreover, we applied this kind of probe to quantitatively detect nucleic acid based on the fluorescence resonance energy transfer system. Results： This method had the linear range 0.025-0.188 μmol/L, with the correlation coefficient of 0.997 and the limit of quantitation of 0.025 μmol/L. Conclusion：The fluorescence resonance energy transfer system between the CdTe quantum dots and ethidium bromide-DNA could be used for quantitative detection of nucleic acid. |
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| Keywords: | DNA quantum dot ethidium bromide fluorescence resonance energy transfer |
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