蜂胶挥发油中高良姜素与白杨素的含量测定

目的:建立蜂胶挥发油中高良姜素与白杨素的含量测定方法。方法:采用高效液相色谱法,选用色谱柱为Agilent ZORBAX Extend(150mm×4.6mm,5μm)C18柱;流动相为甲醇-0.15%磷酸溶液(60∶40),流速为1.0mL/min,检测波长为268nm。结果:高良姜素进样量的质量浓度在0.01031～0.16500μg/mL范围内,线性关系良好(r=0.9996),平均回收率为98.79%,RSD=2.78%;白杨素在0.00938～0.15000μg/mL范围内,线性关系良好(r=0.9998);平均回收率为98.73%,RSD=2.89%。结论:该法简便、快速、准确,重现性好,适用于蜂胶挥发油的质量控制。

Determination of Galangin and Chrysin in Volatile Oil of Propolis

Objective： To establish the determination method of the volatile oil of propolis and chrysin galangin. Methods： HPLC column used for the Agilent ZORBAX Extend （150 mm×4.6 mm, 5 μm） C18 column; mobile phase of methanol-0.15% phosphoric acid （60：40） and flow rate 1.0 mL/min. The detection wavelength was 268 nm. Results： The sample size galangin mass concentration of 0.010-0.165μg/mL range, a good linear relationship（r=0.999 6）, the average recovery was 98.79%, RSD=2.78%; chrysin at 0.009-0.150μg/mL range and good linearity（r=0.999 8）; the average recovery was 98.73%, RSD=2.89%. Conclusion： The method is simple, rapid, accurate and reproducible, Its is suitable for quality control of propolis essential oil.