麝香保心丸对心力衰竭大鼠肺脏肾上腺素受体表达的影响

目的探讨麝香保心丸对心力衰竭大鼠肺脏α_(1A)-肾上腺素受体(α_(1A)-AR)和β_1、β_2肾上腺素受体(β-AR)表达水平的变化。方法选择Wistar大鼠54只,随机分为对照组(A组,10只)、假手术组(B组,10只)、心力衰竭模型对照组(C组,9只)、阳性药物对照组(D组,9只)、麝香保心丸小剂量组(E组,9只)和麝香保心丸大剂量组(F组,9只)。超声心动图检测用药前后大鼠心功能,采用Western blot测定各组大鼠肺组织α_(1A)-AR和β_1-AR、β_2-AR蛋白表达水平。结果用药前,A组与B组大鼠LVEF比较,差异无统计学意义(P>0.05);与B组比较,C、D、E、F组大鼠LVEF明显降低(P<0.01)。用药后与C组比较,F组大鼠LVEF明显改善;与B组比较,C组大鼠α_(1A)-AR、β_1-AR蛋白明显降低,β_2-AR蛋白明显升高;与C组比较,D、E、F组大鼠α_(1A)-AR、β_1-AR、β_2-AR蛋白明显升高(P<0.05,P<0.01)。结论麝香保心丸能改善心肌梗死后心力衰竭大鼠的心脏功能,肾上腺素受体表达水平的变化,有利于维持心力衰竭时肺脏的通气/血流比值,进而对心力衰竭起到有益的治疗作用。

Effect of Shexiangbaoxin Pills on expression of adrenergic receptors in lungs of rats with heart failure

Objective To study the effect of Shexiangbaoxin Pills(MHPP) on expression levels of adrenergic receptorsα_(1A),β_1 andβ_2 adrenergic receptors(AR) in lungs of rats with chronic heart failure(CHF).Methods Fifty four Wistar rats were randomly divided into control group(group A),sham operation group(group B),CHF model group(group C),positive drug control group (group D),low MHPP dose group(group E) and high MHPP dose group(group F),9 in each group.Cardiac function of rats in different groups was monitored by echocardiography before and after MHPP treatment.Expression levels ofα_(1A),β_1 andβ_2 AR in lungs of rats in different groups were measured by Western blot.Results No significant difference was found in the left ventricle ejection faction(LVEF) between groups A and B before MHPP treatment(P>0.05).The LVEF was significantly lower in groups C-F than in group B before MHPP treatment(P<0.01) and significantly higher in group F than in group after MHPP treatment(P<0.05).The expression levels ofα_(1A) andβ_1 AR were significantly lower while that ofβ_2 AR was significantly higher in group C than in group B after MHPP treatment(P<0.05,P<0.01).The expression levels ofα_(1A),β_1 andβ_2 AR were significantly higher in groups D-F than in group C after MHPP treatment(P<0.05, P<0.01).Conclusion MHPP can improve the cardiac function of rats with CHF following myocardial infarction.Adrenergic receptor expression level helps to maintain the lung ventilation/perfusion ratio,thus benefiting the treatment of CHF.