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| 维泰醇对小鼠宫颈癌U14细胞抗肿瘤作用的研究 | |
| 引用本文： | 宿星,巩平,王冬慧,刘文,杨鑫磊,郑秋生.维泰醇对小鼠宫颈癌U14细胞抗肿瘤作用的研究[J].现代肿瘤医学,2012,20(7):1321-1324. |
| 作者姓名： | 宿星 巩平 王冬慧 刘文 杨鑫磊 郑秋生 |
| 作者单位： | 1. 石河子大学医学院,新疆石河子,832000 2. 石河子大学医学院第一附属医院肿瘤内科,新疆石河子,832000 3. 山西省运城市中心医院,山西运城,044000 4. 石河子大学药学院省部共建教育部新疆特种植物药资源重点实验室,新疆石河子,832000 |
| 基金项目： | 科技部重大新药创制科技重大专项(2009ZX09103-141) |
| 摘 要： | 目的:研究维泰醇对宫颈癌U14细胞的抑制增殖与诱导凋亡作用,以探讨维泰醇的抗肿瘤作用机制。方法:采用MTT法检测维泰醇对宫颈癌U14细胞增殖抑制率的影响;采用AO/EB双染色法观察细胞凋亡形态并计算其凋亡率;免疫细胞化学法检测U14细胞内Bcl-2,Bax、Survivin蛋白表达水平。结果:维泰醇对U14细胞增殖有明显的抑制作用,呈剂量及时间依赖性;维泰醇能引起细胞凋亡,并出现典型的凋亡形态。维泰醇下调U14细胞内Bcl-2、Survivin蛋白表达水平,上调Bax蛋白表达水平。结论:维泰醇可抑制宫颈癌U14细胞增殖并诱导细胞凋亡;维泰醇抗宫颈癌的作用机制可能与其下调Bcl-2、Survivin蛋白的表达及上调Bax蛋白的表达有关。 |
| 关 键 词： | 维泰醇 宫颈癌 U14细胞 抗肿瘤 |
Effect of Alternol on the anti-cancer of mouse cervical carcinoma U14 cells |
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| SU Xing , GONG Ping , WANG Donghui , LIU Wen , YANG Xinlei , ZHENG Qiusheng.Effect of Alternol on the anti-cancer of mouse cervical carcinoma U14 cells[J].Journal of Modern Oncology,2012,20(7):1321-1324. | |
| Authors: | SU Xing GONG Ping WANG Donghui LIU Wen YANG Xinlei ZHENG Qiusheng |
| Institution: | 1Shihezi University School of Medicine,Xinjiang Shihezi 832000,China;2Department of Oncology,First Affiliated Hospital of Medical College,Shihezi University,Xinjiang Shihezi 832000,China;3Central Hospital of Yuncheng,Shanxi Yuncheng 044000,China;4Key Laboratory of Xinjiang Endemic Phytomedicine Resources,Ministry of Education,School of Pharmacy,Shihezi University,Xinjiang Shihezi 832000,China. |
| Abstract: | Objective:To investigate the effect of Alternol on the proliferation and apoptosis of mouse cervical carcinoma U14 cells and explore its anti-cancer mechanism.Methods:The inhibitory effect of Alternol on U14 cells was measured by MTT assay.The morphology of apoptotic cells and the apoptotic ratio were observed by acridine orange/ethidium bromide(AO/EB) double staining.The expressions of Bcl-2,Bax and Survivin proteins were observed by immunocytochemistry.Results:Alternol inhibited the proliferation of U14 cells in a dose-and time-dependent manner significantly.Alternol induced apoptosis of U14 cells conspicuously,and appear typical morphology of apoptotic.Alternol could down-regulate the expressions of Bcl-2 and Survivin proteins,and up-regulate the expression of Bax proteins.Conclusion:Alternol inhibits the proliferation and induce apoptosis of cervical carcinoma U14 cells effectively.Alternol down-regulated expression of Bcl-2 and Survivin proteins,and up-regulated expression of Bax proteins maybe related to the mechanisms of anti-cervical carcinoma. |
| Keywords: | Alternol cervical carcinoma U14 cells anti-cancer |
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