| 蒸馏水与稀释碘伏对结肠癌细胞SW480体外增殖的比较研究 |
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| 引用本文： | 庞秋霞,王爱红,刘勇峰,许静洪,陈美霓,赵菊梅. 蒸馏水与稀释碘伏对结肠癌细胞SW480体外增殖的比较研究[J]. 延安大学学报(医学科学版), 2012, 10(1): 1-3 |
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| 作者姓名： | 庞秋霞 王爱红 刘勇峰 许静洪 陈美霓 赵菊梅 |
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| 作者单位： | 1. 延安大学医学院,延安,716000
2. 延安大学附属医院 |
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| 基金项目： | 陕西省卫生厅科研基金资助项目(2008H039);延安市科学技术研究资助项目(2008ks-30) |
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| 摘 要： | 目的研究蒸馏水与稀释碘伏对人结肠癌细胞SW480体外增殖的抑制作用。方法体外培养人结肠癌细胞株SW480,分别用蒸馏水与不同浓度稀释碘伏（0.05%,0.025%,0.01%）处理,MTT（噻唑蓝）法检测蒸馏水与稀释碘伏对SW480细胞的生长影响作用,并进行分析评价。结果 0.01%碘伏处理细胞1 min即可表现出明显的细胞毒性作用,效果远远高于单纯使用蒸馏水的杀伤作用。结论稀释碘伏在体外对人结肠癌细胞SW480的杀伤作用明显高于单纯使用蒸馏水,可望为临床手术中控制腹腔结肠癌细胞种植转移提供临床依据。
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| 关 键 词： | 结肠癌 腹腔灌洗 |
Comparative study of distilled water and diluted povidone-iodione on growth of colon cancer cell line SW480 in vitro |
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| PANG Qiu-xia , WANG Ai-hong , LIU Yong-feng , XU Jing-hong , CHEN Mei-ni , ZHAO Ju-mei. Comparative study of distilled water and diluted povidone-iodione on growth of colon cancer cell line SW480 in vitro[J]. Journal of Yanan University:Medical Science Edition, 2012, 10(1): 1-3 |
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| Authors: | PANG Qiu-xia WANG Ai-hong LIU Yong-feng XU Jing-hong CHEN Mei-ni ZHAO Ju-mei |
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| Affiliation: | 1.Medical College of Yan’an University; 2.First Affiliated Hospital of Yan’an University,Yan’an shaanxi 716000,China) |
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| Abstract: | Objective To study the inhibition effect of distilled water and diluted Povidone-iodione on growth of human colon Cancer cell line SW480 Methods The Colon Cancer cell line SW480 cells were treated with distilled water and different concentrations(0.05%,0.025%,0.01%) of diluted povidone-iodine,MTT assay was used to examine cellular proliferation.Results SW480 cells were killed significantly after cells were treated by 0.01% povidone-iodine for about 1 min,The killing effect of 0.01% povidone-iodine is much higher than the pure distilled water.Conclusion Diluted povidone-iodine has a significant killing effect on human colon cancer cell SW480 in vitro,which would provide a clinical basis to control abdominal metastasis of colon cancer cells on clinical surgery. |
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