壮骨止痛胶囊有效部位在大鼠体内肠襻吸收状态的研究

目的 通过对壮骨止痛胶囊有效部位在体肠吸收状态的研究,为壮骨止痛胶囊的质量控制及有效成分的分离提供依据.方法 利用高效液相色谱法(HPLC)构建有效部位的HPLC图谱,然后采用SD大鼠在体肠襻吸收实验,构建大鼠在体肠襻吸收后的HPLC图谱,通过各时点的HPLC图谱比较,确定被小肠吸收的差异峰.结果 壮骨止痛胶囊无水乙醇部位中有5个主要化学成分可通过肠襻吸收.结论 壮骨止痛胶囊有效部位体内肠襻吸收实验可指导壮骨止痛胶囊化学成分的分离.

Study on rat intestinal loop absorption of active fractions in Zhuanggu Zhitong Capsule

Objective To provide the basis for separation analysis of active ingredients and quality control of Zhuanggu Zhitong Capsule(ZZC) by the study on intestinal loop absorption of active fractions in ZZC.Methods To construct the spectrum of active fractions by HPLC.Then,the HPLC spectra of post-absorption were constructed by absorption experiment of intestinal loop in SD rats.The different peaks of intestinal loop absorption could be determined by comparing the HPLC spectra in different points.Results The five chemical compositions on absolute ethyl alcohol in ZZC could be absorbed through intestinal loop.Conclusion The experiment of intestinal loop absorption can instruct the separation of chemical compositions in ZZC.