Isolation and detection of phorbolesters in crotonoil with HPLC. |
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| Authors: | Bauer R Tittel G Wagner H |
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| Affiliation: | Institut für Pharmazeutische Biologie der Universit?t München. |
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| Abstract: | A HPLC method using reversed-phase columns is described for the determination and isolation of phorbolesters in crotonoil. The identification of the compounds is based on an off-line combination of HPLC-MS, as well as on fatty acid analysis by GC. With this method several new phorbolesters of crotonoil could be detected. This method is also suitable for the determination of phorbol- or similar diterpenesters in other Euphorbiaceae. |
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