缺锌大鼠海马胆囊收缩素和一氧化氮合酶阳性细胞数目的变化

目的　观察缺锌对海马胆囊收缩素 (CCK)和一氧化氮合酶 (NOS)阳性细胞数的影响及其与动物行为变化的关系 ,研究缺锌对学习记忆影响的机制。方法　通过管饲低锌液体饲料复制缺锌大鼠模型。二周后 ,使用Y 迷宫实验检测动物学习记忆能力 ;采用ABC免疫组化法和NADPH d组化检测海马各区CCK和NOS阳性细胞数目。结果　与对照组相比 ,缺锌大鼠学习记忆能力明显下降 (P <0 0 5 ) ;缺锌大鼠血清锌水平明显降低 (P <0 0 5 ) ,但海马锌含量未见明显改变 ;缺锌大鼠海马CA1区和CA3区CCK和NOS阳性细胞数目均明显减少。结论　缺锌导致海马CA1和CA3区CCK和NOS阳性细胞数目减少可能是缺锌影响脑学习记忆功能的重要机制之一

Changes of cholecystokinin and nitric oxide synthase positive neurons of hippocampus in zinc-deficiency rat

Objective To investigate the relationship between the effect of zinc deficiency on cholecystokinin(CCK) and nitric oxide synthase(NOS) positive neurons in rats hippocampus and changes of animal behavior, and to analyze the mechanism of zinc deficiency on learning memory. Methods Zinc deficiency rat models were duplicated by intubation feeding low-zinc liquid for two weeks. Y-maze test was applied to study the learning and memory ability in rats; NADPH-d histochemistry method and ABC immunohistochemistry were used to investigate changes of NOS and CCK positive neurons in different hippocampal subfields. Results Compared with the control group, the learning and memory ability of zinc deficiency rats was significantly decreased (P<0.05); the serum zinc content was obviously reduced(P<0.05), no change of hippocampal zinc content was found; the number of CCK and NOS positive neurons in hippocampal CA1 and CA3 area of zinc deficiency rats were significantly decreased(P<0.05). Conclusion Zinc deficiency may damage the learning-memory ability of the rats; the effects might be related to the low of CCK and NOS positive neurons in hippocampal CA1 and CA3 area in zinc deficiency rats.